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高速原子間力顕微鏡による結晶性セルロース酵素分解のリアルタイム1分子イメージング
https://doi.org/10.24517/00010438
https://doi.org/10.24517/00010438333ed2f0-2c36-4774-809c-d28c1d1870b2
名前 / ファイル | ライセンス | アクション |
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SC-PR-UHIHASHI-T-140.pdf (1.5 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2017-10-03 | |||||
タイトル | ||||||
タイトル | 高速原子間力顕微鏡による結晶性セルロース酵素分解のリアルタイム1分子イメージング | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Real Time Single Molecular Imaging of Enzymatic Degradation of Crystalline Cellulose by High-speed Atomic Force Microscopy | |||||
言語 | ||||||
言語 | jpn | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
ID登録 | ||||||
ID登録 | 10.24517/00010438 | |||||
ID登録タイプ | JaLC | |||||
著者 |
五十嵐, 圭日子
× 五十嵐, 圭日子× 内橋, 貴之× 鮫島, 正浩× 安藤, 敏夫 |
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著者別表示 |
Igarashi, Kiyohiko
× Igarashi, Kiyohiko× Uchihashi, Takayuki× Samejima, Masahiro× Ando, Toshio |
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提供者所属 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 金沢大学ナノ生命科学研究所 / 金沢大学理工研究域数物科学系 | |||||
書誌情報 |
生物物理 = Biophysics 巻 53, 号 3, p. 140-144, 発行日 2013-01-01 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0582-4052 | |||||
NCID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA11511277 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.2142/biophys.53.140 | |||||
出版者 | ||||||
出版者 | 日本生物物理学会 = The Biophysical Society of Japan | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | We carried out the real-time visualization of individual cellulase molecules using a high-speed atomic force microscopy (HS-AFM), having sub-second time resolution and nanometer space resolution. Trichoderma reesei cellobiohydrolase I (TrCel7A) molecules were observed to slide unidirectionally along the crystalline cellulose surface, and the catalytic domain without the cellulose-binding domain moved with a velocity similar to that of the intact TrCel7A enzyme although inactive mutant did not move, indicating a processive movement of the enzyme driven by the hydrolytic reaction. We also observed traffic jam of enzyme molecules and synergistic degradation of crystalline cellulose using HS-AFM. | |||||
権利 | ||||||
権利情報 | Copyright © 日本生物物理学会 | The Biophysical Society of Japan | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | http://www.biophys.jp/ | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://www.jstage.jst.go.jp/browse/biophys/-char/ja/ | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | http://scpj.tulips.tsukuba.ac.jp/info/nii.html |