WEKO3
インデックスリンク
アイテム
{"_buckets": {"deposit": "adc33076-b02d-4855-9c9c-cb9548d3a8f4"}, "_deposit": {"created_by": 3, "id": "10764", "owners": [3], "pid": {"revision_id": 0, "type": "depid", "value": "10764"}, "status": "published"}, "_oai": {"id": "oai:kanazawa-u.repo.nii.ac.jp:00010764", "sets": ["937"]}, "author_link": ["16446", "16447", "138", "145", "16445", "16448", "16444"], "item_4_biblio_info_8": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2014-01-01", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "1-2", "bibliographicPageEnd": "72", "bibliographicPageStart": "65", "bibliographicVolumeNumber": "78", "bibliographic_titles": [{"bibliographic_title": "Chromatographia"}]}]}, "item_4_description_21": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "A new fluorescent-based high-performance liquid chromatography (HPLC) assay using 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole (NBD-C1) was employed to determine iron (Fe) bioavailability to red tide phytoplankton in seawater. After growing four red tide species (Prymnesium parvum, Heterosigma akashiwo, Eutreptiella gymnastica, and Oltmannsiellopsis viridis) in f/2 artificial seawater under different Fe conditions, soluble extracts of the phytoplankton were derivatized using different fluorescent reagents (NBD-C1, 4-fluoro-7-nitrobenzo-2-oxa-1,3-diazole; NBD-F, fluorescamine, and ortho-phthalaldehyde; OPA) followed by HPLC assay. Among the four fluorescent reagents, NBD-C1 was most effective for derivatizing the phytoplankton extracts which would consist of proteins and peptides. HPLC chromatograms of the NBD-derivatized extracts showed gradual changes (decrease/increase) of six peaks for different Fe conditions. Four of the peaks decreased, while two peaks increased with the increase of Fe concentrations in the culture medium. Considering the consistency and sensitivity of chromatogram peaks E and A to different Fe, phosphate and nitrate conditions for all phytoplankton studied, the ratio of these two peaks (IE/A) has been proposed as the indicator of Fe bioavailability to red tide phytoplankton.", "subitem_description_type": "Abstract"}]}, "item_4_description_22": {"attribute_name": "内容記述", "attribute_value_mlt": [{"subitem_description": "In Press", "subitem_description_type": "Other"}]}, "item_4_publisher_17": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Friedr. Vieweg und Sohn Verlags GmbH / Springer Verlag"}]}, "item_4_relation_12": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type": "isVersionOf", "subitem_relation_type_id": {"subitem_relation_type_id_text": "10.1007/s10337-014-2792-8", "subitem_relation_type_select": "DOI"}}]}, "item_4_source_id_11": {"attribute_name": "NCID", "attribute_value_mlt": [{"subitem_source_identifier": "AA10680676", "subitem_source_identifier_type": "NCID"}]}, "item_4_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0009-5893", "subitem_source_identifier_type": "ISSN"}]}, "item_4_version_type_25": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_ab4af688f83e57aa", "subitem_version_type": "AM"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Hasegawa, Hiroshi"}], "nameIdentifiers": [{"nameIdentifier": "145", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "90253335", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=90253335"}, {"nameIdentifier": "90253335", "nameIdentifierScheme": "金沢大学研究者情報", "nameIdentifierURI": "http://ridb.kanazawa-u.ac.jp/public/detail.php?kaken=90253335"}, {"nameIdentifier": "90253335", "nameIdentifierScheme": "研究者番号", "nameIdentifierURI": "https://nrid.nii.ac.jp/nrid/1000090253335"}]}, {"creatorNames": [{"creatorName": "Nakagawa, K."}], "nameIdentifiers": [{"nameIdentifier": "16444", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Rahman, Mohammad Azizur"}], "nameIdentifiers": [{"nameIdentifier": "16445", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Takemura M."}], "nameIdentifiers": [{"nameIdentifier": "16446", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Maki, Teruya"}], "nameIdentifiers": [{"nameIdentifier": "138", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "70345601", "nameIdentifierScheme": "金沢大学研究者情報", "nameIdentifierURI": "http://ridb.kanazawa-u.ac.jp/public/detail.php?kaken=70345601"}, {"nameIdentifier": "70345601", "nameIdentifierScheme": "研究者番号", "nameIdentifierURI": "https://nrid.nii.ac.jp/nrid/1000070345601"}]}, {"creatorNames": [{"creatorName": "Naito, K."}], "nameIdentifiers": [{"nameIdentifier": "16447", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Rahman, Mahmuda Mamunur"}], "nameIdentifiers": [{"nameIdentifier": "16448", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2017-10-03"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "SC-PR-HASEGAWA-H-65.pdf", "filesize": [{"value": "754.0 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 754000.0, "url": {"label": "SC-PR-HASEGAWA-H-65.pdf", "url": "https://kanazawa-u.repo.nii.ac.jp/record/10764/files/SC-PR-HASEGAWA-H-65.pdf"}, "version_id": "4430a993-53c0-4d0b-85ce-2d00a6b7e8ab"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "Fluorescent-based HPLC assay", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Iron bioavailability", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Marine phytoplankton", "subitem_subject_scheme": "Other"}, {"subitem_subject": "NBD-C1", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Red tide", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "A Fluorescent-Based HPLC Assay Using 4-Chloro-7-nitrobenzo-2-oxa-1, 3-diazole as Derivatization Agent for the Determination of Iron Bioavailability to Red Tide Phytoplankton", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "A Fluorescent-Based HPLC Assay Using 4-Chloro-7-nitrobenzo-2-oxa-1, 3-diazole as Derivatization Agent for the Determination of Iron Bioavailability to Red Tide Phytoplankton"}]}, "item_type_id": "4", "owner": "3", "path": ["937"], "permalink_uri": "http://hdl.handle.net/2297/40176", "pubdate": {"attribute_name": "公開日", "attribute_value": "2017-10-03"}, "publish_date": "2017-10-03", "publish_status": "0", "recid": "10764", "relation": {}, "relation_version_is_last": true, "title": ["A Fluorescent-Based HPLC Assay Using 4-Chloro-7-nitrobenzo-2-oxa-1, 3-diazole as Derivatization Agent for the Determination of Iron Bioavailability to Red Tide Phytoplankton"], "weko_shared_id": -1}
A Fluorescent-Based HPLC Assay Using 4-Chloro-7-nitrobenzo-2-oxa-1, 3-diazole as Derivatization Agent for the Determination of Iron Bioavailability to Red Tide Phytoplankton
http://hdl.handle.net/2297/40176
http://hdl.handle.net/2297/40176608a598e-9e30-4982-b88a-296be219a1da
名前 / ファイル | ライセンス | アクション |
---|---|---|
SC-PR-HASEGAWA-H-65.pdf (754.0 kB)
|
|
Item type | 学術雑誌論文 / Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2017-10-03 | |||||
タイトル | ||||||
タイトル | A Fluorescent-Based HPLC Assay Using 4-Chloro-7-nitrobenzo-2-oxa-1, 3-diazole as Derivatization Agent for the Determination of Iron Bioavailability to Red Tide Phytoplankton | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Hasegawa, Hiroshi
× Hasegawa, Hiroshi× Nakagawa, K.× Rahman, Mohammad Azizur× Takemura M.× Maki, Teruya× Naito, K.× Rahman, Mahmuda Mamunur |
|||||
書誌情報 |
Chromatographia 巻 78, 号 1-2, p. 65-72, 発行日 2014-01-01 |
|||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0009-5893 | |||||
NCID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA10680676 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1007/s10337-014-2792-8 | |||||
出版者 | ||||||
出版者 | Friedr. Vieweg und Sohn Verlags GmbH / Springer Verlag | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | A new fluorescent-based high-performance liquid chromatography (HPLC) assay using 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole (NBD-C1) was employed to determine iron (Fe) bioavailability to red tide phytoplankton in seawater. After growing four red tide species (Prymnesium parvum, Heterosigma akashiwo, Eutreptiella gymnastica, and Oltmannsiellopsis viridis) in f/2 artificial seawater under different Fe conditions, soluble extracts of the phytoplankton were derivatized using different fluorescent reagents (NBD-C1, 4-fluoro-7-nitrobenzo-2-oxa-1,3-diazole; NBD-F, fluorescamine, and ortho-phthalaldehyde; OPA) followed by HPLC assay. Among the four fluorescent reagents, NBD-C1 was most effective for derivatizing the phytoplankton extracts which would consist of proteins and peptides. HPLC chromatograms of the NBD-derivatized extracts showed gradual changes (decrease/increase) of six peaks for different Fe conditions. Four of the peaks decreased, while two peaks increased with the increase of Fe concentrations in the culture medium. Considering the consistency and sensitivity of chromatogram peaks E and A to different Fe, phosphate and nitrate conditions for all phytoplankton studied, the ratio of these two peaks (IE/A) has been proposed as the indicator of Fe bioavailability to red tide phytoplankton. | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | In Press | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa |