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Regulation of Cytochrome b5 Expression by miR-223 in Human Liver: Effects on Cytochrome P450 Activities
https://doi.org/10.24517/00015109
https://doi.org/10.24517/000151092ba6c103-87b7-41db-9437-fc972e7623cc
名前 / ファイル | ライセンス | アクション |
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PH-PR-NAKAJIMA-M-780.pdf (617.5 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2017-10-04 | |||||
タイトル | ||||||
タイトル | Regulation of Cytochrome b5 Expression by miR-223 in Human Liver: Effects on Cytochrome P450 Activities | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
ID登録 | ||||||
ID登録 | 10.24517/00015109 | |||||
ID登録タイプ | JaLC | |||||
著者 |
Takahashi, Kei
× Takahashi, Kei× Oda, Yuki× Toyoda, Yasuyuki× Fukami, Tatsuki× Yokoi, Tsuyoshi× Nakajima, Miki |
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著者別表示 |
深見, 達基
× 深見, 達基× 横井, 毅× 中嶋, 美紀 |
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提供者所属 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 金沢大学ナノ生命科学研究所 / 金沢大学医薬保健研究域薬学系 | |||||
書誌情報 |
Pharmaceutical Research 巻 31, 号 3, p. 780-794, 発行日 2014-03-01 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0724-8741 | |||||
NCID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA10632083 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1007/s11095-013-1200-7 | |||||
出版者 | ||||||
出版者 | Springer | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Purpose: Cytochrome b 5 (b 5) is a hemoprotein that transfers electrons to several enzymes to fulfill functions in fatty acid desaturation, methemoglobin reduction, steroidogenesis, and drug metabolism. Despite the importance of b 5, the regulation of b 5 expression in human liver remains largely unknown. We investigated whether microRNA (miRNA) might be involved in the regulation of human b 5. Methods: Twenty-four human liver specimens were used for correlation analysis. In silico analysis and luciferase assay were performed to determine whether the predicted miRNAs functionally target to b 5. The miR-223 was overexpressed into HepG2 cells infected with adenovirus expressing human cytochrome P450. Results: In human livers, the b 5 protein levels were not positively correlated with the b 5 mRNA levels, and miR-223 levels were inversely correlated with the b 5 mRNA levels or the translational efficiencies. The luciferase assay showed that miR-223 functionally binds to the element in the 3′-untranslated region of b 5 mRNA. The overexpression of miR-223 significantly reduced the endogenous b 5 protein level and the mRNA stability in HepG2 cells. Moreover, the overexpression of miR-223 significantly reduced CYP3A4-catalyzed testosterone 6β-hydroxylation activity and CYP2E1-catalyzed chlorzoxazone 6-hydroxylase activity but not CYP1A2-catalyzed 7-ethoxyresorufin O-deethylase activity. Conclusions: miR-223 down-regulates b 5 expression in the human liver, modulating P450 activities. © 2013 Springer Science+Business Media New York. | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa |