A 45-year-old man who had refractory right chylothorax after esophagectomy for esophageal cancer, underwent lymphoscintigraphy with Tc-99m human serum albumin. Focal abnormal uptake was seen in the mid-abdomen on planar image 30 minutes after the tracer injection. SPECT/CT delineated the extent of the accumulation between the anastomotic site and the right pleural effusion area. SPECT/CT effectively detected the site of lymphatic leakage.