ヒト末梢血単核球由来の血管内皮前駆細胞(EPC)marker陽性細胞を分離、mRNAの発現解析等を実施。KDR陽性細胞が少数であることを証明。培養にてコロニー形成するEPCの出現を観察し、質的解析を実施。EPC同定評価に必要な実験系の確立を行い、一定の成果を得た。同時に、数種類のステントを作成し、大型動物(ブタ)冠動脈にステントを留置、その効果の検証を重ねた。EPC捕捉STENT留置群においては、新生内膜の増殖が抑制されうる傾向にあるものと推測された。急性血栓閉塞を発症した症例はなく、安全性のあるデバイスに成りうる可能性が示唆された。
The positive cell of human endothelial progenitor cell (EPS) markers were isolated from peripheral blood mononuclear cell and were analyzed mRNA level by real time RT-PCR. There were few KDR positive cells into the human mononuclear cell. Endothelial colony formation cells (early EPS) colonies were identified on day 14 to 21 after it were cultured. We assessed immunophenotyping of early EPC using fluorescence microscope and characterized the early EPS by non quantitative RT-PCR. We established method of experiment for identification of EPSs. Then, anesthetized domestic swine (weight 25±5 kg, 2 months old) were implanted 25 EPC-capture stents (n=13) and 25 polymer-coated stents (n=13) into left coronary artery. The arteries were harvested at 14 days after the stenting. Histological analysis was performed by hematoxylin-eosin staining and stent strut surface were assessed by electron microscopy. In morphometric analysis, each layer of vascular wall was calculated in the section. Procedural success was achieved in all swine and all stents were patent without thrombosis. Electron microscopy suggest that uniformly endothelialization at the stent strut surface, and hematoxylin-eosin staining showed that neointimal thickining were thin and uniformly.
血管内皮前駆細胞選択的捕捉VEGF固定化ステントの開発に関する研究
.png)
ME-PR-YAMAGISHI-M-kaken 2011-5p.pdf
(388.13KB)
