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ヘモグロビンのアロステリック効果
https://doi.org/10.24517/00034923
https://doi.org/10.24517/00034923a8e8330e-1dc1-4fe3-803c-1f4f39915d85
名前 / ファイル | ライセンス | アクション |
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ME-PR-MAWATARI-K-kaken 1988-50p.pdf (1.1 MB)
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Item type | 報告書 / Research Paper(1) | |||||
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公開日 | 2017-10-05 | |||||
タイトル | ||||||
タイトル | ヘモグロビンのアロステリック効果 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | The allosteric effect in hemoglobin | |||||
言語 | ||||||
言語 | jpn | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_18ws | |||||
資源タイプ | research report | |||||
ID登録 | ||||||
ID登録 | 10.24517/00034923 | |||||
ID登録タイプ | JaLC | |||||
著者 |
森本, 英樹
× 森本, 英樹× 今井, 清博× 梶田, 昭彦× 北川, 禎三× 郷, 信広× 高橋, 征三× 松川, 茂× 馬渡, 一浩× 小西, 康子× 根矢, 三郎× 今泉, 和彦× 浅井, 博 |
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書誌情報 |
昭和62(1987)年度 科学研究費補助金 総合研究(A) 研究成果報告書 en : 1987 Fiscal Year Final Research Report Summary 巻 1985-1987, p. 50p., 発行日 1988-03-01 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | 1)色々な手段で, デオキシヘモグロビンの酸素結合の親和性を変化させたとき, ヘモグロビン分子内各部位の構造変化のおこり方を調べると, ヘム近傍の構造変化と, それ以外のグロビン部分の構造変化は, 2状態モデルの期待に反して, 同期していないことがわかった. 2)4つの金属ポルフィリンがデオキシヘムのモデルとして使えることがわかった. その中で,Ni-プロトポルフィリンが一番よく研究され, 通常のヘモグロビンの中ばかりでなく, 化学修飾されたヘモグロビンの中でもよいデオキシヘムのモデルとなっていることがわかった. 3)Ni-プロトポルフィリンを使ったヘモグロビンの酸素結合の中間段階のモデルは, 2状態モデルから期待されるような状態から, いちじるしくはずれることがわかった. 4)ヘムのFe^<2+>を, 鉄属遷移金属イオンに置換することでFe^<2+>の電子状態とグロビンとの相互作用が調べられた. 置換のグロビンの構造に対する影響は周期律表に非常によくしたがって変化することがわかった. この結果を充分説明する考えはまだ, 得られていない. 5)α1β1相互作用が重要であることを示唆するデータが得られた. 6)α89ヒスチジンが, ヘモグロビンのアルカリボーア効果に約25%寄与していることがわかった. 7)大腸菌の遺伝子を操作することによって, 任意の一次構造をもったヘモグロビンを作ることができるようになった. 8)紫外のレーザーを励起光とする共鳴ラマンスペクトル測定装置が作られ, ヘモグロビンに使われはじめた. 9)コンピュータによる, 立体構造エネルギー関数法で, ミオグロビンの動的構造が解析された. | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | 1) The conformational changes of various parts ot a deoxyhemoglobin molecule were investigated when its oxygen affinity was altered by use of abnormal hemoglobins and chemically modified ones. It was found that the conformational changes around heme and those of the globin moiety did not take place in concerted manner on the contrary to the expectation of the two state allosteric model. 2) Four metalloprotoporphyrins were found to medel the deoxyheme. Among them Ni-protoporphyrinIX has been studied most extensively. Judging from the oxygen equilibrium properties of Ni-Fe hybrid hemoglobins. Ni-protoporphyrinIX behaves just like "frozen" deoxyheme not only in unmodified hemoglobins but also in modified ones. 3) The model for the intermediate state of the oxygenation of hemoglobin prepared by use of Ni-protoporphyrin showed a conformational state, which could not be accomodated in the framework of the two state allosteric model. 4) The interaction between the electronic state of iron and the globin moiety was investigated by substituteing iron serries transition metal ions for iron. The conformational state of the hemoglobin having the metalloprotoporphyrin changed according to the position of the element in the periodical table. No complete explanation of the result has been given. 5) Data were obtained to suggest the importance of the 1 1 interaction. 6) 89 Histidine was shown to contribute about 25% of the alkaline Bohr effect. 7) It became possible to prepare a hemoglobin having any primary structure by gene manipulation of E. coli. 8) An equipment to measure a resonance Raman spectra excited by ultraviolet laser beams was constructed. 9) The dynamic three dimentional structure of myoglobin was analyed by the computer calculation of the conformational energy function. | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 研究課題/領域番号: 60304099, 研究期間(年度):1985–1987 | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 出典:「ヘモグロビンのアロステリック効果」研究成果報告書 課題番号60304099 (KAKEN:科学研究費助成事業データベース(国立情報学研究所)) 本文データは著者版報告書より作成 |
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著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-60304099/ | |||||
関連名称 | https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-60304099/ | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-60304099/603040991987kenkyu_seika_hokoku_gaiyo/ | |||||
関連名称 | https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-60304099/603040991987kenkyu_seika_hokoku_gaiyo/ | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://kaken.nii.ac.jp/search/?qm=50135050 | |||||
関連名称 | https://kaken.nii.ac.jp/search/?qm=50135050 |