This study "environmental impact assessment method for coast using fertilized eggs of Sargassum horneri" was carried out in four years from April 2018 to March 2022. First, the effects of pH and salinity on fertilized eggs of Sargassum horneri were investigated in detail, and a standard protocol for culturing fertilized eggs of Sargassum horneri was established. Subsequently, representative harmful chemical substances like heavy metals were selected, fertilized eggs of Sargassum horneriwere cultured according to the established standard protocol, and susceptibility data of Sargassum horneri to each chemical substance was collected. Furthermore, by comparing with susceptibility data of other seaweeds, it was clarified that fertilized eggs of Sargassum horneri are particularly sensitive to copper and that chronic growth inhibition by copper is likely to occur.
本研究「アカモク受精卵を用いた沿岸海域環境影響評価手法の開発と評価」は、2018年4月から2022年3月までの4ヵ年で実施した。まず,アカモク受精卵に対するpHや塩分の影響を詳細に調査し,アカモク受精卵の培養に関する標準プロトコルを確立した。続いて代表的な有害化学物質(重金属、アンモニア)を選定し,確立した標準プロトコルにしたがってアカモク受精卵を培養し,各化学物質に対するアカモクの感受性データを採取した.さらに他の海藻類の感受性データと比較検証し,アカモク受精卵が特に銅に対して感受性が高く,銅による慢性的な生長阻害が生じやすいことを明らかにした.