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脳脊髄液中のアミロイドβオリゴマー化抑制物質解明と早期診断・治療法開発の展開
https://doi.org/10.24517/00059329
https://doi.org/10.24517/00059329a2cea524-a248-4bfc-bf07-b27744be1e02
名前 / ファイル | ライセンス | アクション |
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ME-PR-IKEDA-T-kaken 2018-4p.pdf (281.1 kB)
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Item type | 報告書 / Research Paper(1) | |||||
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公開日 | 2020-10-19 | |||||
タイトル | ||||||
タイトル | 脳脊髄液中のアミロイドβオリゴマー化抑制物質解明と早期診断・治療法開発の展開 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Inhibitors of amyloid beta oligomerization in human cerebrospinal fluid | |||||
言語 | ||||||
言語 | jpn | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_18ws | |||||
資源タイプ | research report | |||||
ID登録 | ||||||
ID登録 | 10.24517/00059329 | |||||
ID登録タイプ | JaLC | |||||
著者別表示 |
Ikeda, Tokuhei
× Ikeda, Tokuhei |
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提供者所属 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 金沢大学医薬保健研究域医学系 | |||||
書誌情報 |
平成29(2017)年度 科学研究費補助金 若手研究(B) 研究成果報告書 en : 2017 Fiscal Year Final Research Report 巻 2016-04-01 – 2018-03-31, p. 4p., 発行日 2018-06-06 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | ヒト脳脊髄液のAmyloidβ蛋白に対するオリゴマー化抑制作用の原因物質の解明を行った。脳脊髄液を液体クロマトグラフィー(Size-exclusion column)で分注し、MS/MSで評価し、Angiotensin I、II、IIIの他、既知の蛋白の断片などの16種のペプチドの合成を行った。オリゴマー化の抑制力をPICUP法で評価し、AngiotensinI-IIIを含む11種のペプチドで抑制力をみとめ、Aβの線維化に対する抑制力をThioflavin T法を用いて評価し、9種のペプチドで抑制力を認めた。これらの蛋白はAlzheimer病の発生機序の究明、治療法の開発に寄与する。 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | The purpose of this research was to identify inhibitors of amyloid β (Aβ) oligomerization in human cerebrospinal fluid (CSF). CSF was fractionated using centrifugation through a column and size-exclusion high-performance liquid chromatography. Two CSF fractions had inhibitory effects on Aβ oligomerization, as determined using tandem mass spectrometry. We identified 16 peptides with the desired activity: Angiotensins I, II, and III, and known fragments of these peptides. Using photo-induced cross-linking of unmodified proteins (PICUP), we identified 11 peptides with Aβ oligomerization inhibitory activity, including Angiotensins I-III. Thioflavin T fluorescence was used to identify 9 peptides with Aβ fibrillation inhibitory activity, including Angiotensins I-III. The discovery of these peptides contributes to the investigation of the mechanism underlying Alzheimer's disease pathology and the development of therapeutic strategies for Alzheimer’s disease. | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 研究課題/領域番号:16K19506, 研究期間(年度):2016-04-01 – 2018-03-31 | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 出典:研究課題「脳脊髄液中のアミロイドβオリゴマー化抑制物質解明と早期診断・治療法開発の展開」課題番号16K19506 (KAKEN:科学研究費助成事業データベース(国立情報学研究所)) (https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-16K19506/16K19506seika/)を加工して作成 |
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著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://kaken.nii.ac.jp/ja/search/?kw=30755773 | |||||
関連名称 | https://kaken.nii.ac.jp/ja/search/?kw=30755773 | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-16K19506/ | |||||
関連名称 | https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-16K19506/ | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-16K19506/16K19506seika/ | |||||
関連名称 | https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-16K19506/16K19506seika/ |