WEKO3
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アイテム
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Racemic [125I]OIDV was separated into its two optical isomers (-)-[125I]OIDV and (+)-[125I]OIDV by HPLC. To investigate VAChT binding affinity (Ki) of two OIDV isomers, in vitro binding assays were performed. In vivo biodistribution study of each [125I]OIDV isomer in blood, brain regions and major organs of rats was performed at 2,30 and 60 min post-injection. In vivo blocking study were performed to reveal the binding selectivity of two [125I]OIDV isomers to VAChT in vivo. Ex vivo autoradiography were performed to reveal the regional brain distribution of two [125I]OIDV isomers and (-)-[123I]OIDV for SPECT at 60 min postinjection.\n\nResults\nVAChT binding affinity (Ki) of (-)-[125I]OIDV and (+)-[125I]OIDV was 22.1 nM and 79.0 nM, respectively. At 2 min post-injection, accumulation of (-)-[125I]OIDV was the same as that of (+)-[125I]OIDV. However, (+)-[125I]OIDV clearance from the brain was faster than (-)-[125I]OIDV. 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In vivo differences between two optical isomers of radioiodinated o-iodo-transdecalinvesamicol for Use as a radioligand for the vesicular acetylcholine transporter
https://doi.org/10.24517/00065239
https://doi.org/10.24517/00065239ae3453f8-2eec-41bf-8c21-c8abcac4b001
名前 / ファイル | ライセンス | アクション |
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ME-PR-SHIBA-K-0146719.pdf (297.2 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2022-01-28 | |||||
タイトル | ||||||
タイトル | In vivo differences between two optical isomers of radioiodinated o-iodo-transdecalinvesamicol for Use as a radioligand for the vesicular acetylcholine transporter | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
ID登録 | ||||||
ID登録 | 10.24517/00065239 | |||||
ID登録タイプ | JaLC | |||||
著者 |
Uno, Izumi
× Uno, Izumi× Kozaka, Takashi× Miwa, Daisuke× Kitamura, Yoji× Azim, Mohammad Anwar-ul× Ogawa, Kazuma× Taki, Junichi× Kinuya, Seigo× Shiba, Kazuhiro |
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著者別表示 |
小阪, 孝史
× 小阪, 孝史× 北村, 暘二× 小川, 数馬× 瀧, 淳一× 絹谷, 清剛× 柴, 和弘 |
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提供者所属 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 金沢大学疾患モデル総合研究センター | |||||
書誌情報 |
PLoS ONE 巻 11, 号 1, p. e0146719, 発行日 2016-01-11 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1932-6203 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1371/journal.pone.0146719 | |||||
出版者 | ||||||
出版者 | Public Library of Science | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Purpose To develop a superior VAChT imaging probe for SPECT, radiolabeled (-)-OIDV and (+)-OIDV were isolated and investigated for differences in their binding affinity and selectivity to VAChT, as well as their in vivo activities. Procedures Radioiodinated o-iodo-trans-decalinvesamicol ([125I]OIDV) has a high binding affinity for vesicular acetylcholine transporter (VAChT) both in vitro and in vivo. Racemic [125I]OIDV was separated into its two optical isomers (-)-[125I]OIDV and (+)-[125I]OIDV by HPLC. To investigate VAChT binding affinity (Ki) of two OIDV isomers, in vitro binding assays were performed. In vivo biodistribution study of each [125I]OIDV isomer in blood, brain regions and major organs of rats was performed at 2,30 and 60 min post-injection. In vivo blocking study were performed to reveal the binding selectivity of two [125I]OIDV isomers to VAChT in vivo. Ex vivo autoradiography were performed to reveal the regional brain distribution of two [125I]OIDV isomers and (-)-[123I]OIDV for SPECT at 60 min postinjection. Results VAChT binding affinity (Ki) of (-)-[125I]OIDV and (+)-[125I]OIDV was 22.1 nM and 79.0 nM, respectively. At 2 min post-injection, accumulation of (-)-[125I]OIDV was the same as that of (+)-[125I]OIDV. However, (+)-[125I]OIDV clearance from the brain was faster than (-)-[125I]OIDV. At 30 min post-injection, accumulation of (-)-[125I]OIDV (0.62 ± 0.10%ID/g) was higher than (+)-[125I]OIDV (0.46 ± 0.07%ID/g) in the cortex. Inhibition of OIDV binding showed that (-)-[125I]OIDV was selectively accumulated in regions known to express VAChT in the rat brain, and ex vivo autoradiography further confirmed these results showing similar accumulation of (-)-[125I]OIDV in these regions. Furthermore, (-)-[123I]OIDV for SPECT showed the same regional brain distribution as (-)-[125I]OIDV. Conclusion These results suggest that radioiodinated (-)-OIDV may be a potentially useful tool for studying presynaptic cholinergic neurons in the brain. |
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内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | CC-BY 4.0 | |||||
権利 | ||||||
権利情報 | Copyright © 2016 Uno et al.This is an open access article distributed under the terms of the Creative Commons Attribution License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited., Published by the Public Library of Science | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | http://www.plosone.org/ | |||||
関連名称 | http://www.plosone.org/ | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0146719 | |||||
関連名称 | https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0146719 |