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Quantification of myoglobin deoxygenation and intracellular partial pressure of O2 during muscle contraction during haemoglobin-free medium perfusion
http://hdl.handle.net/2297/24446
http://hdl.handle.net/2297/24446b44df77b-aa9e-4393-91ee-13c0c4c9d093
名前 / ファイル | ライセンス | アクション |
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ED-PR-MASUDA-K-630.pdf (1.5 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2017-10-02 | |||||
タイトル | ||||||
タイトル | Quantification of myoglobin deoxygenation and intracellular partial pressure of O2 during muscle contraction during haemoglobin-free medium perfusion | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Takakura, Hisashi
× Takakura, Hisashi× Masuda, Kazumi× Hashimoto, Takeshi× Iwase, Satoshi× Jue, Thomas |
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提供者所属 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 金沢大学人間社会研究域人間科学系 | |||||
書誌情報 |
Experimental Physiology 巻 95, 号 5, p. 630-640, 発行日 2010-05-01 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0958-0670 | |||||
NCID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA10751559 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1113/expphysiol.2009.050344 | |||||
出版者 | ||||||
出版者 | Wiley-Blackwell | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Although the O2 gradient regulates O2 flux from the capillary into the myocyte to meet the energy demands of contracting muscle, intracellular O2 dynamics during muscle contraction remain unclear. Our hindlimb perfusion model allows the determination of intracellular myoglobin (Mb) saturation () and intracellular oxygen tension of myoglobin () in contracting muscle using near infrared spectroscopy (NIRS). The hindlimb of male Wistar rats was perfused from the abdominal aorta with a well-oxygenated haemoglobin-free Krebs-Henseleit buffer. The deoxygenated Mb (Δ[deoxy-Mb]) signal was monitored by NIRS. Based on the value of Δ[deoxy-Mb], and were calculated, and the time course was evaluated by an exponential function model. Both and started to decrease immediately after the onset of contraction. The steady-state values of and progressively decreased with relative work intensity or muscle oxygen consumption. At the maximal twitch rate, and were 49% and 2.4 mmHg, respectively. Moreover, the rate of release of O2 from Mb at the onset of contraction increased with muscle oxygen consumption. These results suggest that at the onset of muscle contraction, Mb supplies O2 during the steep decline in, which expands the O2 gradient to increase the O2 flux to meet the increased energy demands. © 2010 The Physiological Society. | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa |