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Nitrate reductase from the magnetotactic bacterium Magnetospirillum magnetotacticum MS-1: Purification and sequence analyses
https://doi.org/10.24517/00010182
https://doi.org/10.24517/00010182beda56cb-ae36-44c6-916f-4b186bf3e62c
名前 / ファイル | ライセンス | アクション |
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SC-PR-TAOKA-A-197.pdf (1.0 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2017-10-03 | |||||
タイトル | ||||||
タイトル | Nitrate reductase from the magnetotactic bacterium Magnetospirillum magnetotacticum MS-1: Purification and sequence analyses | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
ID登録 | ||||||
ID登録 | 10.24517/00010182 | |||||
ID登録タイプ | JaLC | |||||
著者 |
Taoka, Azuma
× Taoka, Azuma× Yoshimatsu, Katsuhiko× Kanemori, Masaaki× Fukumori, Yoshihiro |
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著者別表示 |
田岡 , 東
× 田岡 , 東× 金森, 正明× 福森, 義宏 |
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提供者所属 | ||||||
内容記述タイプ | Other | |||||
内容記述 | ベンチャー・ビジネス・ラボラトリー | |||||
提供者所属 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 金沢大学大学院自然科学研究科動態生理学 | |||||
書誌情報 |
Canadian Journal of Microbiology 巻 49, 号 3, p. 197-206, 発行日 2003-01-01 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0008-4166 | |||||
NCID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA00597703 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1139/w03-028 | |||||
出版者 | ||||||
出版者 | National Research Council Canada | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | We purified the nitrate reductase from the soluble fraction of Magnetospirillum magnetotacticum MS-1. The enzyme was composed of 86- and 17-kDa subunits and contained molybdenum, non-heme iron, and heme c. These properties are very similar to those of the periplasmic nitrate reductase found in Paracoccus pantotrophus. The M. magnetotacticum nap locus was clustered in seven open reading frames, napFDAGHBC. The phylogenetic analyses of NapA, NapB, and NapC suggested a close relationship between M. magnetotacticum nap genes and Escherichia coli nap genes, which is not consistent with the 16S rDNA data. This is the first finding that the ホア subclass of Proteobacteria possesses a napFDAGHBC-type nap gene cluster. The nap gene cluster had putative fumarate and nitrate reduction regulatory protein (Fnr) and NarL protein binding sites. Furthermore, we investigated the effect of molybdate deficiency in medium on the total iron content of the magnetosome fraction and discussed the physiological function of nitrate reductase in relation to the magnetite synthesis in M. magnetotacticum. | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |