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Purification, characterization and sequence analyses of the extracellular giant hemoglobin from Oligobrachia mashikoi
https://doi.org/10.24517/00010214
https://doi.org/10.24517/000102147b8fa450-d1f3-429f-b8c1-c10a357fbf0b
名前 / ファイル | ライセンス | アクション |
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SC-PR-FUKUMORI-Y-283.pdf (1.2 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2017-10-03 | |||||
タイトル | ||||||
タイトル | Purification, characterization and sequence analyses of the extracellular giant hemoglobin from Oligobrachia mashikoi | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
ID登録 | ||||||
ID登録 | 10.24517/00010214 | |||||
ID登録タイプ | JaLC | |||||
著者 |
Nakagawa, Taro
× Nakagawa, Taro× Onoda, Seiko× Kanemori, Masaaki× Sasayama, Yuichi× Fukumori, Yoshihiro |
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著者別表示 |
中川, 太郎
× 中川, 太郎× 金森, 正明× 笹山, 雄一× 福森, 義宏 |
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提供者所属 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 金沢大学理工研究域自然システム学系 | |||||
書誌情報 |
Zoological Science 巻 22, 号 3, p. 383-291, 発行日 2005-03-01 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0289-0003 | |||||
NCID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA10545874 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.2108/zsj.22.283 | |||||
出版者 | ||||||
出版者 | 日本動物学会 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | We purified an extracellular hemoglobin with the molecular mass of ca. 440 kDa from the whole homogenates of Oligobrachia mashikoi (phylum Pogonophora) by a one-step gel-filtration. The preparation was pure to be crystallized. The P50 values of the hemoglobin and the fresh blood prepared from O. mashikoi were about 0.82 Torr and 0.9 Torr, respectively, which were much lower than the P50 value of human hemoglobin. However, the n values of the hemoglobin and the blood were about 1.2 and 1.1, respectively. Using the improved tricine SDS-PAGE, we could separate O. mashikoi hemoglobin into four kinds of the globin chains, A1, A2, B1 and B2, and succeeded for the first time in cloning and sequencing of the complete cDNA encoding B1 globin gene, in addition to A1, A2 and B2 globin genes in full length. We found that all globin genes have the extracellular signal sequences in each molecule and the distal His of the B1 globin chain is replaced to Gln. Finally, we constructed phylogenetic trees of the hemoglobins from Pogonophora, Vestimentifera and Annelida. © 2005 Zoological Society of Japan. | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |