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Thiol modulation of the chloroplast ATP synthase is dependent on the energization of thylakoid membranes
https://doi.org/10.24517/00010489
https://doi.org/10.24517/00010489676f44eb-0b6f-405e-8cbf-a014e706d0d8
名前 / ファイル | ライセンス | アクション |
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SC-PR-KONNO-H-626.pdf (567.5 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2017-10-03 | |||||
タイトル | ||||||
タイトル | Thiol modulation of the chloroplast ATP synthase is dependent on the energization of thylakoid membranes | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
ID登録 | ||||||
ID登録 | 10.24517/00010489 | |||||
ID登録タイプ | JaLC | |||||
著者 |
Konno, Hiroki
× Konno, Hiroki× Nakane, Takeshi× Yoshida, Masasuke× Ueoka-Nakanishi, Hanayo× Hara, Satoshi× Hisabori, Toru |
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著者別表示 |
紺野, 宏記
× 紺野, 宏記 |
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提供者所属 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 金沢大学ナノ生命科学研究所 / 金沢大学理工研究域生命理工学系 | |||||
書誌情報 |
Plant and Cell Physiology 巻 53, 号 4, p. 626-634, 発行日 2012-04-01 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0032-0781 | |||||
NCID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA0077511X | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1093/pcp/pcs018 | |||||
出版者 | ||||||
出版者 | Oxford University Press (OUP) / Japanese Society of Plant Physiologists 日本植物生理学会 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Thiol modulation of the chloroplast ATP synthase γ subunit has been recognized as an important regulatory system for the activation of ATP hydrolysis activity, although the physiological significance of this regulation system remains poorly characterized. Since the membrane potential required by this enzyme to initiate ATP synthesis for the reduced enzyme is lower than that needed for the oxidized form, reduction of this enzyme was interpreted as effective regulation for efficient photophosphorylation. However, no concrete evidence has been obtained to date relating to the timing and mode of chloroplast ATP synthase reduction and oxidation in green plants. In this study, thorough analysis of the redox state of regulatory cysteines of the chloroplast ATP synthase γ subunit in intact chloroplasts and leaves shows that thiol modulation of this enzyme is pivotal in prohibiting futile ATP hydrolysis activity in the dark. However, the physiological importance of efficient ATP synthesis driven by the reduced enzyme in the light could not be demonstrated. In addition, we investigated the significance of the electrochemical proton gradient in reducing the γ subunit by the reduced form of thioredoxin in chloroplasts, providing strong insights into the molecular mechanisms underlying the formation and reduction of the disulfide bond on the γ subunit in vivo. © 2012 The Author. | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa |