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Heme Orientation of Cavity Mutant Hemoglobins (His F8 → Gly) in Either α or β Subunits: Circular Dichroism, 1H NMR, and Resonance Raman Studies
https://doi.org/10.24517/00014412
https://doi.org/10.24517/0001441242bc5bf2-f149-48e3-b3ea-a061e2223466
名前 / ファイル | ライセンス | アクション |
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ME-PR-NAGAI-M-585.pdf (742.0 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2017-12-05 | |||||
タイトル | ||||||
タイトル | Heme Orientation of Cavity Mutant Hemoglobins (His F8 → Gly) in Either α or β Subunits: Circular Dichroism, 1H NMR, and Resonance Raman Studies | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
ID登録 | ||||||
ID登録 | 10.24517/00014412 | |||||
ID登録タイプ | JaLC | |||||
著者 |
Nagai, Masako
× Nagai, Masako× Nagai, Yukifumi× Aki, Yayoi× Sakurai, Hiroshi× Mizusawa, Naoki× Ogura, Takashi× Kitagawa, Teizo× Yamamoto, Yasuhiko× Nagatomo, Shigenor |
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著者別表示 |
長井, 雅子
× 長井, 雅子× 桜井, 博× 山本, 泰彦 |
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書誌情報 |
Chirality 号 1 August 2016, p. 585-592, 発行日 2016-08-01 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0899-0042 | |||||
NCID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA10678277 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1002/chir.22620 | |||||
出版者 | ||||||
出版者 | John Wiley and Sons Inc. | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Native human adult hemoglobin (Hb A) has mostly normal orientation of heme, whereas recombinant Hb A (rHb A) expressed in E. coli contains both normal and reversed orientations of heme. Hb A with the normal heme exhibits positive circular dichroism (CD) bands at both the Soret and 260-nm regions, while rHb A with the reversed heme shows a negative Soret and decreased 260-nm CD bands. In order to examine involvement of the proximal histidine (His F8) of either α or β subunits in determining the heme orientation, we prepared two cavity mutant Hbs, rHb(αH87G) and rHb(βH92G), with substitution of glycine for His F8 in the presence of imidazole. CD spectra of both cavity mutant Hbs did not show a negative Soret band, but instead exhibited positive bands with strong intensity at the both Soret and 260-nm regions, suggesting that the reversed heme scarcely exists in the cavity mutant Hbs. We confirmed by 1H NMR and resonance Raman (RR) spectroscopies that the cavity mutant Hbs have mainly the normal heme orientation in both the mutated and native subunits. These results indicate that the heme Fe-His F8 linkage in both α and β subunits influences the heme orientation, and that the heme orientation of one type of subunit is related to the heme orientation of the complementary subunits to be the same. The present study showed that CD and RR spectroscopies also provided powerful tools for the examination of the heme rotational disorder of Hb A, in addition to the usual 1H NMR technique. Chirality 28:585–592, 2016. © 2016 Wiley Periodicals, Inc. © 2016 Wiley Periodicals, Inc. | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Embargo Period 24 months | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa |