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Preparation and Characterization of a Polyclonal Antibody against Brominated Protein
http://hdl.handle.net/2297/39191
http://hdl.handle.net/2297/39191eea60fce-832b-429b-bc1e-f63449d4f98a
名前 / ファイル | ライセンス | アクション |
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ME-PR-KAMBAYASHI-Y-95.pdf (429.5 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2017-10-03 | |||||
タイトル | ||||||
タイトル | Preparation and Characterization of a Polyclonal Antibody against Brominated Protein | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Kambayashi, Yasuhiro
× Kambayashi, Yasuhiro× Ogino, Keiki× Takemoto, Kei× Imagama, Takashi× Takigawa, Tomoko× Kimura, Shingo× Hibino, Yuri× Hitomi, Yoshiaki× Nakamura, Hiroyuki |
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書誌情報 |
Journal of Clinical Biochemistry and Nutrition 巻 44, 号 1, p. 95-103, 発行日 2009-01-01 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0912-0009 | |||||
NCID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA10710201 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.3164/jcbn.08-196 | |||||
出版者 | ||||||
出版者 | The Editorial Secretariat of JCBN = 日本酸化ストレス学会 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | (Di)bromotyrosine is formed by the specific reaction of eosinophil peroxidase and can be used as an eosinophil activation marker. In the present study, an antibody for (di)bromotyrosine in proteins was prepared to investigate the pathogenesis of eosinophil-related diseases such as allergic responses. A rabbit polyclonal antibody was raised against brominated keyhole limpet hemocyanin. The specificity of the antiserum was investigated with an enzyme-linked immunosorbent assay (ELISA). The antiserum recognized brominated bovine serum albumin (BSA) and dibromotyrosine-conjugated BSA. The antiserum also reacted with chlorinated BSA and di-iodotyrosine-conjugated BSA. Moreover, the specificity of the antiserum was investigated using competitive ELISA. Dibromotyrosine and di-iodotyrosine inhibited the recognition of brominated BSA by the antiserum. However, the recognition of brominated BSA by the antiserum was not inhibited by bromotyrosine, chlorotyrosine, iodotyrosine, nitrotyrosine, aminotyrosine, phosphotyrosine, or tyrosine. These results suggested that the epitope of the antiserum is dihalogenated tyrosine. Immunohistochemically, the antiserum stained brominated rat eosinophils but not chlorinated or nitrated eosinophils. In conclusion, an antiserum for dihalogenated protein was prepared. It is expected that the antiserum will be useful for the analysis of the pathogenesis of allergic diseases such as asthma and atopic dermatitis. | |||||
権利 | ||||||
権利情報 | Copyright © The Editorial Secretariat of JCBN 日本酸化ストレス学会 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://www.jstage.jst.go.jp/browse/jcbn | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | http://sfrrj.umin.jp/JCBN.htm |