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重油のエストロゲン作用とその評価法 (<特集>環境ホルモンと分析化学)
http://hdl.handle.net/2297/25012
http://hdl.handle.net/2297/250125369bd64-ac1d-4a8c-8e2f-c34bacf75a81
名前 / ファイル | ライセンス | アクション |
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PH-PR-HAYAKAWA-K-617.pdf (634.2 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2017-10-04 | |||||
タイトル | ||||||
タイトル | 重油のエストロゲン作用とその評価法 (<特集>環境ホルモンと分析化学) | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Estrogenic activity of heavy oil and its assay method (<Special Article>Endocrine Disrupting Chemicals and Analytical Chemistry) | |||||
言語 | ||||||
言語 | jpn | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
木津, 良一
× 木津, 良一× 加藤, 詳子× 薄井, 修× 早川, 和一 |
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提供者所属 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 金沢大学医薬保健研究域薬学系 | |||||
書誌情報 |
分析化学 = Japan analyst 巻 48, 号 6, p. 617-622, 発行日 1999-05-05 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0525-1931 | |||||
NCID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN00222633 | |||||
出版者 | ||||||
出版者 | The Japan Society for Analytical Chemistry = 日本分析化学会 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | エストロゲン作用の新しいin vitroアッセイ法として, ヒト乳がん由来MCF-7細胞におけるプロゲステロン受容体(PgR)の発現量を指標とする方法を提案し, 現在最もはん用されてぃるE-Screenアッセイ法と比較するとともに, C重油のエストロゲン作用を検討した. 試料としては, 重油のエタノール抽出物を用いた. PgRの発現量をウエスタンブロット法で測定したところ, 17β-エストラジオール(E_2)処理によりPgRの発現量が高まり, 更にE_2アンタゴニストであるタモキシフェンの処理を加えるとPgRの発現量は減少した. E-Screenアッセイにおいても, E_2及びタモキシフェン処理に対して類似の応答を示し, PgR発現量がエストロゲン作用の良い指標であることが明らかになった. 次に, 重油抽出物のエストロゲン作用について検討した. E_2非存在下では重油抽出物の処理でわずかながらPgRの発現量が高まったが, E_2存在下ではPgRの発現量は減少した. 以上の結果から, 重油中には弱いエストロゲン作用を示し, E_2アンタゴニストとして作用する化合物が含まれることが明らかとなった. また, 重油抽出物の処理では死細胞数の増加が観察された. 重油や環境試料のように細胞死を引き起こす成分を含む可能性がある試料については, 本研究のPgRの発現量を指標とする方法が適していると考えられた. The authors have proposed a new in vitro assay method for estrogenicity. The cellular progesterone receptor (PgR) level in the human breast cancer cell line MCF-7 was measured because PgR has been known to be upregulated by 17β-estradiol (E_2). The cellular PgR level was measured by a western blotting technique. While an E_2 treatment increased the PgR level, tamoxyfen, a typical E_2 antagonist decreased the PgR level elevated by the E_2 treatment. These results revealed that the cellular PgR level is a good indication to evaluate the estrogenicity. Next, the estrogenic activities of Nakhodka heavy oil and a commercial C-heavy oil were examined. Heavy oil crude extracts prepared with ethanol were subjected to the assay. While the extracts increased the PgR level under the E_2-free condition, the extracts decreased the PgR level elevated by the E_2 treatment. These results indicate that the heavy oil contains compounds which show weak estrogenic activity and act as E_2 antagonists. Dead cells were observed after the oil extract treatment, and their number increased with an increase of oil extract concentration in the cell-culture medium. Environmental samples such as oil-extract, river water, and air particulate extract comprise a wide variety of compounds, and are thought to have a cell-killing effect. A cellular PgR expression method is considered to be more appropriate for assessing the estrogenic activities of environmental samples, compared with the existing assay methods, such as the cell-proliferation method (E-Screen assay) and the reporter-gene methods. | |||||
権利 | ||||||
権利情報 | Copyright (c) 1999 日本分析化学会 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | http://www.jsac.or.jp/index.html | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | http://ci.nii.ac.jp/naid/110002905735/en/ |