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田七人参成分のサイトカイン(IL-1β)分泌抑制作用
http://hdl.handle.net/2297/42853
http://hdl.handle.net/2297/42853156cf909-aa29-44cd-ad31-ccec4f3dc198
名前 / ファイル | ライセンス | アクション |
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PH-PR-SHIMADA-T-191.pdf (6.5 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2017-10-04 | |||||
タイトル | ||||||
タイトル | 田七人参成分のサイトカイン(IL-1β)分泌抑制作用 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Inhibitory effect of Panax notoginseng methanol extract (denchi) on the cytokine IL-1β release from PBMC cells | |||||
言語 | ||||||
言語 | jpn | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
嶋田, 努
× 嶋田, 努× 丸山, 博文× 栗原, 久× 丸山, 悠司× 赤瀬, 智子× 油田, 正樹 |
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書誌情報 |
薬理と治療 = Basic pharmacology and therapeutics 巻 36, 号 3, p. 191-198, 発行日 2008-03-01 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0386-3603 | |||||
NCID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN00242029 | |||||
出版者 | ||||||
出版者 | ライフサイエンス出版 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | 本研究では, 田七人参(田七)の抗炎症作用の機序および活性成分の解明を目的に, 炎症と関係の深い8種類のサイトカイン(INF-γ, TNF-α, IL-1β, IL-2, IL-4, IL-5, IL-6およびIL-10)の分泌に対する作用を検討した. 田七のメタノール抽出エキス(田七エキス(A):100μg/mL)は, それぞれIL-1β分泌を88%, TNF-α分泌を51%, IL-4分泌を34%, INF-γ分泌を28%, IL-10分泌を27%, IL-6分泌を13%抑制した. 他方, IL-2分泌には有意な影響を認めず, IL-5分泌を48%促進した. そこで分泌阻害効果がもっとも強かったIL-1β分泌についてさらに検討したところ, 田七エキス(A)の阻害率は, 30μg/mLで17%, および100μg/mLで78%であった. 一方, シリカゲル薄層クロマトグラフィー(展開溶媒としてCHCl3:MeOH:H2O=7:3:0.5混合液, 展開距離15cm)でginsenoside Rg1およびそれよりRf値の低い画分(B)(人参と共通するginsenoside類を多く含む画分)のIL-1β分泌阻害率は, 200μg/mLの高濃度でも20%であり, きわめて低いことがわかった. しかし, Rf値の高い画分(C)はそのIC50が2.86μ/mLと, 有意なIL-1β分泌阻害作用を示すことが認められた. 本結果は, 田七の抗炎症作用が主としてIL-1βの分泌阻害作用を介して発現すること, その活性成分は人参に含まれない脂溶性の高いginsenoside類に起因することが強く示唆された. The aim of this research was to find the mechanism of anti-inflammatory effect and the properties of the active constituents of methanol extract of Panax notoginseng (Denchi) in vitro. Denchi (100 μg/mL) inhibited the release of IL-1/8 (88%), INF-α (51%), IL-4 (34%), INF-γ (28%), IL-10 (27%), IL-6 (13%) from PMBC. However, it did not change the IL-2 release, and increased the IL-5 release (48%). The fraction-C, in which denchi-specific ginsenosides were contained, showed strong and dose-related inhibition of IL-1β release (IC50 = 2.86 μg/mL). However, the inhibitory activity of Panax ginseng-related ginsenosides (fraction-B) was extremely weak, 20% at 200 μg/mL, as compared with that of the fraction-C. These results suggest that the anti-inflammatory effect of Denchi is achieved through an inhibition of the IL-1β release, and that the active compounds are highly lipid-soluble ginsenosides. | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |