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Human telomerase exists in two distinct active complexes in vivo
http://hdl.handle.net/2297/14551
http://hdl.handle.net/2297/145511aaf650f-ef90-4c48-a162-a4c9c55cc4a0
名前 / ファイル | ライセンス | アクション |
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CA-PR-MURAKAMI-S-641.pdf (265.8 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2017-10-05 | |||||
タイトル | ||||||
タイトル | Human telomerase exists in two distinct active complexes in vivo | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Mizuno, Hideki
× Mizuno, Hideki× Khurts, Shilagardi× Seki, Takahiko× Hirota, Yasuhide× Kaneko, Shuichi× Murakami, Seishi |
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提供者所属 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 金沢大学がん研究所 | |||||
書誌情報 |
Journal of Biochemistry 巻 141, 号 5, p. 641-652, 発行日 2007-05-01 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0021-924X | |||||
NCID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA00694073 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1093/jb/mvm071 | |||||
出版者 | ||||||
出版者 | 日本生化学会 = Japanese Biochemical Society | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Telomerase, a stable complex of telomerase reverse transcriptase (TERT) and template RNA (TERC), is responsible for telomere maintenance. During purification trials of recombinant human telomerase of the two components reconstituted in insect cells, we identified two complexes of human telomerase of molecular masses 680 and 380 kDa, both of which retain telomerase activity in vitro. We show here that the former complex does not include Hsp90 (heat shock protein 90) and its telomerase activity is resistant to Hsp90 inhibitors, whereas the latter contains Hsp90 and its telomerase activity is sensitive to Hsp90 inhibitors. N-terminal of FLAG-hTERT in the former is exposed, as this complex was efficiently purified with anti-FLAG M2 affinity resin. We also identified two different telomerase complexes in HeLa cells, in addition to ectopically expressed hTERT. Most of endogenous hTERT and FLAG-hTERT was detected around 680 kDa. These two complexes in HeLa cells have the same properties as their respective reconstituted telomerases. The unstable property of the telomerase complex with Hsp90, especially in the presence of Hsp90 inhibitors, was due to proteasome-mediated degradation of hTERT, since proteasome inhibitors prevented hTERT degradation in vivo. To our knowledge, this is the first demonstration of two distinct active complexes of human telomerase ectopically expressed in insect and mammalian cells. © 2007 The Japanese Biochemical Society. | |||||
権利 | ||||||
権利情報 | © 2007 The Japanese Biochemical Society. | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
関連URI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | http://dx.doi.org/10.1093/jb/mvm071 | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | http://jb.oxfordjournals.org/cgi/content/abstract/141/5/641 |