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Multiple factors influencing the release of hTERT mRNA from pancreatic cancer cell lines in in vitro culture
http://hdl.handle.net/2297/32228
http://hdl.handle.net/2297/322289c5be62a-67c1-4c83-90a7-fd4856a1708e
名前 / ファイル | ライセンス | アクション |
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CA-PR-OHTSUBO-K-545.pdf (167.9 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2017-10-05 | |||||
タイトル | ||||||
タイトル | Multiple factors influencing the release of hTERT mRNA from pancreatic cancer cell lines in in vitro culture | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Okada, Gensaku
× Okada, Gensaku× Watanabe, Hiroyuki× Ohtsubo, Koushiro× Mouri, Hisatsugu× Yamaguchi, Yasushi× Motoo, Yoshiharu× Sawabu, Norio |
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書誌情報 |
Cell Biology International 巻 36, 号 6, p. 545-553, 発行日 2012-06-01 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1065-6995 | |||||
NCID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA10882014 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1042/CBI20090471 | |||||
出版者 | ||||||
出版者 | International Federation for Cell Biology / Portland Press | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Since telomerase expression is highly prevalent in human cancers, the quantitation of serum/plasma hTERT (human telomerase reverse transcriptase) mRNA levels may be useful for early detection of PCa (pancreatic cancer). To analyse the correspondence between exhTERT (extracellular hTERT) mRNA levels and hTERT expression, we designed a cell culture system to investigate factors modulating the extracellular levels of hTERT mRNA in media conditioned by eight PCa cell lines. We found that the level of exhTERT mRNA was dependent on cell growth rate. MIAPaCa-2, PANC-1, KLM-1 and PK-9 cells expressed high levels of exhTERT mRNA, independent of cell density, whereas proliferating PK-59, BxPC-3 and PK-45H cells released low levels of exhTERT mRNA. The augmented release of mRNA by spontaneous dead MIAPaCa-2 cells was further increased at postconfluence. In Capan-1 cells, low correspondence of marker was also due to RNase secretion. Upon reaching confluence, some PCa cell lines showed down-regulation of hTERT expression. Following cell-cell adhesion, as shown by E-cadherin engagement, PK-59 cells showed levels of extracellular message below the limits of detection, a loss not due to an increase in message degradation. These results suggest that the levels of exhTERT mRNA in the medium of PCa cell lines are altered not only in response to cell growth rate and cell destruction, but are responsive to extracellular cues such as RNases and cell density. A cell-free assay for exhTERT mRNA may therefore not be useful for early detection of PCa. © The Author(s) Journal compilation © 2012 International Federation for Cell Biology. | |||||
権利 | ||||||
権利情報 | © 2012 International Federation for Cell Biology | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa |