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親和吸収と自動電気泳動によるM成分416検体の同定
http://hdl.handle.net/2297/45605
http://hdl.handle.net/2297/45605cce60a10-ab76-426b-81fc-e0a2387d5328
名前 / ファイル | ライセンス | アクション |
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CA-PR-MIGITA-S-1992.pdf (5.4 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2017-10-05 | |||||
タイトル | ||||||
タイトル | 親和吸収と自動電気泳動によるM成分416検体の同定 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Easily classified immunoglobulin M-component of 416 sera by affinity absorption and automatic cellulose acetate electrophoresis | |||||
言語 | ||||||
言語 | jpn | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
右田, 俊介
× 右田, 俊介× 竹上, 美也子× 奥村, 次郎× 永井, 康雄× 橋本, 寿美子× 大竹, 皓子× 芝, 紀代子× 小西, 奎子× 瀬崎, 達雄 |
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提供者所属 | ||||||
内容記述タイプ | Other | |||||
内容記述 | がん研究所 | |||||
書誌情報 |
生物物理化学 = The physico-chemical biology 巻 36, 号 6, p. 387-394, 発行日 1992-01-01 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0031-9082 | |||||
NCID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN00129729 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.2198/sbk.36.387 | |||||
出版者 | ||||||
出版者 | 電気泳動学会 = Japanese Electrophoresis Society | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | M成分の同定用の免疫電気泳動または免疫固定法には欠点が多い. 親和吸収電気泳動ではクラス特異的試薬として Omnibind, Jacalin, dithiothreitol を3本のチップにとって凍結乾燥した. これに試料血清を吸引して, サンプルトレーに入れ全自動電気泳動すると, どのチップによりM成分のパタンが変化したかにより, IgG, IgA, IgMを同定した. この方法は全自動泳動装置があれば短時間 (40分) で, 初めての人でも容易にタイピングすることができた. そこでM成分血清416例を分析し, 384例, 92.3%について正しく同定できた. 残りは比較的稀なIgA2, IgD, L鎖型, H鎖型などで, 特定のパタンにはならなかった. さらに微量のM成分は判定に困難な場合があった. そのほか実施する上での注意点をのべた. この方法は抗体を使わないM成分タイピングであり, 経済的でルーチンの検査法として広く用いられてもいいと思われる. M-components of immunoglobulin in the sera have been classified by immunoelectrophoresis, a delicate and time consuming procedure. A new method, affinity absorbed electrophoresis, was described with advantages of easy, rapid (40min) and economical procedure. Instead of antisera, Omnibind A/G, Jacalin and dithiothreitol as specific reagents for IgG, IgA and IgM, respectively, were taken into three microtips and lyophilized. Each 10μl sera was taken into these tips and then applied for automatic electrophoresis. Either disappearance, or decreased concentration, or more rapid or slower migration of M-component than original was estimated as positive reaction with the reagents. Then the heavy-chain classes of M-component were discriminated. Three hundred eighty-four M-components out of 416 sera (92.3%) were easily identified. Corresponding patterns of the other 32 sera containing IgD, IgA2, L-chain and heavy-chain M-component, respectively, were also described, but not distinctive. However, affinity absorbed electrophoresis is good to identify M-component class as a routine analysis. | |||||
権利 | ||||||
権利情報 | Copyright © 日本電気泳動学会 Japanese Electrophoresis Society | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | http://www.jes1950.jp/ | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://www.jstage.jst.go.jp/browse/sbk/-char/ja/ |