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Activation of MMP-9 by membrane type-1 MMP/MMP-2 axis stimulates tumor metastasis
http://hdl.handle.net/2297/47887
http://hdl.handle.net/2297/47887fa0fbb5e-c8f8-493b-9f61-24496c23f795
名前 / ファイル | ライセンス | アクション |
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CA-PR-SATO-H-347.pdf (907.5 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2017-10-05 | |||||
タイトル | ||||||
タイトル | Activation of MMP-9 by membrane type-1 MMP/MMP-2 axis stimulates tumor metastasis | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Li, Zichen
× Li, Zichen× Takino, Takahisa× Endo, Yoshio× Sato, Hiroshi |
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書誌情報 |
Cancer Science 巻 108, 号 3, p. 347-353, 発行日 2017-03-01 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1347-9032 | |||||
NCID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA11808050 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1111/cas.13134 | |||||
出版者 | ||||||
出版者 | Japanese Cancer Association = 日本癌学会 / John Wiley & Sons | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | An artificial receptor for proMMP-9 was created by fusing tissue inhibitor of MMP-1 (TIMP-1) with type II transmembrane mosaic serine protease (MSP-T1). Expression of MSP-T1 in 293T cells induced binding of proMMP-9, which was processed by MMP-2 activated by membrane type 1 MMP (MT1-MMP). HT1080 cells transfected with the MSP-T1 gene produced activated MMP-9 in collagen gel, and addition of proMMP-2 to the culture augmented it, which resulted in intensive collagen digestion. These cells metastasized into chick embryonic liver more than control cells. Treatment of HT1080 cells with concanavalin A in the presence of exogenous proMMP-2 induced activation of not only proMMP-2 but also proMMP-9. Knockdown of MT1-MMP or TIMP-2 expression with siRNA suppressed activation of both proMMP-2 and proMMP-9. Transfection of TIMP-1 siRNA suppressed cell binding and activation of proMMP-9, but not proMMP-2 activation. Knockdown of a disintegrin and metalloproteinase 10 (ADAM10) expression reduced cell binding and processing of proMMP-9. These results suggest that proMMP-9, which binds to a receptor complex containing TIMP-1 and ADAM10, is activated by the MT1-MMP/MMP-2 axis, and MMP-9 thus activated stimulates cellular proteolysis and metastasis. © 2016 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association. | |||||
権利 | ||||||
権利情報 | Copyright © 2016 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association. | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | http://www.jca.gr.jp/ | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | http://onlinelibrary.wiley.com/journal/10.1111/%28ISSN%291349-7006 |