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Therefore, the complete clarification of human cardiac nerves are difficult article.\nIn order to resolve this difficulty, we applied new specific technology. 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全胚免疫染色と分子生物学的方法による内臓と四肢の神経・血管・筋の形成と再生の解明
http://hdl.handle.net/2297/48789
http://hdl.handle.net/2297/48789ef06c988-63f6-4d4a-ba16-195f393ec4e6
名前 / ファイル | ライセンス | アクション |
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ME-PR-TANAKA-S-kaken 2006-7p.pdf (234.5 kB)
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Item type | 報告書 / Research Paper(1) | |||||
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公開日 | 2017-10-05 | |||||
タイトル | ||||||
タイトル | 全胚免疫染色と分子生物学的方法による内臓と四肢の神経・血管・筋の形成と再生の解明 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Deep observational researches regarding the profound structures, morphogenesis and regeneration of nerves, bloods and muscles existing in the visceral internal organs as well as four limbs, using sui genesis technology of whole-mount staining, and molecular biology | |||||
言語 | ||||||
言語 | jpn | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_18ws | |||||
資源タイプ | research report | |||||
著者 |
田中, 重徳
× 田中, 重徳 |
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著者別表示 |
Tanaka, Shigenori
× Tanaka, Shigenori |
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書誌情報 |
平成17(2005)年度科学研究費補助金 基盤研究(C) 研究報告書 en : 2005 Fiscal Year Final Research Report 巻 2004-2005, p. 7p., 発行日 2006-04-01 |
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出版者 | ||||||
出版者 | 金沢大学大学院医学系研究科 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | 心臓に分布する交感神経、即ち心臓神経の形態解明の研究を推進した。心臓神経は椎骨に接する交感神経幹から広範囲に亘り多数起こり、神経同士の結合が多く、走行が複雑であるので、内蔵直接伴行の迷走神経由来の心臓枝よりも形態解明は困難である。とりわけ、ヒトの心臓神経は実験脊椎動物よりも形態が大きく、脂肪が多いので形態解明は至難である。本研究では、アルコールによる脱脂、アリザリン・レッドによる神経染色、そしてエチレン・グリコールによる血管形態明示という方法を開発し、心臓神経と心臓・血管の詳細な形態を広領域に解明した。そして、ヒトのみの固有観点ではなく、脊椎動物総合形態学と発生学的観点をも加えての全貌的意義付けを学会講演にて発表し、掲載された(第8回臨床解剖研究会と第16回日本末梢神経学会)。又、心臓枝と心臓神経の形態に関する国際学術雑誌の投稿原稿を加筆・修正している。形態形成に関しては、マウスとラットよりもヒトの形態に近いジャコウネズミにおいて、心臓神経の形態と形態形成解明を全胚免疫染色を用いて行い、成果を第110回日本解剖学会で発表し、国際学術雑誌投稿準備中である(博士課程学生の提出論文)。ジャコウネズミ肝臓の発生と臨床医学的意味(Endoclinology 2004 : Aant. Enbryol. 2004)と口蓋帆挙筋分布神経と下顎神経の形態についても解明した(Clin Anat 2004 ; Surg Radiol Anat 2004)。肝鎌状間膜と肝円策、そして臍ヒダ経由の肝臓実質に分布する動・静脈と神経の形態と形態形成、そして胸骨剣状突起周囲のリンパ管と肝臓との関係解明をヒトとジャコウネズミにおいて行い、発生学意義の再検討と臨床医学との連携を深めている。又、骨異常の測定・診断と治療方法の解明の共同研究と頭・頚の筋形成に関しての分子組織化学的研究を進めている。 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Researches of elucidation of form and structure of the sympathetic nerve distributed in the heart, that is, cardiac nerves were furthermore promoted. The cardiac nerves were found to arise numerous in number from very broad areas of the sympathetic trunk, communicating each other very numerously. Furthermore, their patterns or forms of running were much more complicated than those of the cardiac branches of the vagus nerve. As a result, clarification of features of these cardiac nerves are difficult than the cardiac branches of the vagus nerve. Inter alia, human examples applicable for researches are exceedingly large as those of other vertebrate and further more, they communicate each other and their coursing figures are much more complicated than those of the cardiac branches of the vagus nerve. Therefore, the complete clarification of human cardiac nerves are difficult article. In order to resolve this difficulty, we applied new specific technology. Using this technology, we are able to grease alcohol by using alcohol, stain nerves with arizarin red S, and to elucidate blood vessels in all experimental examples of humans and other vertebrates. In consequence, the cardiac nerves were found to arise from the sympathetic trunk in a wide area from the cervical until the lowest thoracic level, i.e., ThI0 (and further more ThI2) in human examples. Furthermore, the three-dimensional structures of human cadavers have been clarified fundamentally not only from the human anatomical point of view, but also comprehensively from the vertebrate comparative anatomy and embryology. We have been made presentations of these results in academic conferences (the 8^<th> Meeting of Japanese Research Society of Clinical Anatomy and the 16^<th> Meeting of Japanese Research Society of Periphera Nerve). Therewithal, the contribution manuscripts of the international science magazine concerning the form of the cardiac nerves and branches are being to retouched and corrected. The clarification of morphology and morphogenesis of the cardiac nerves are performed by using methods for whole embryo immunity staining in muskrats which are nearer human's form than the mouse and the rat for the form formation (doctor's course student's submitting thesis). These results of this research were presented in the 110th in Japanese Anatomy Association. Contribution to the international science magazine is also being prepared. The form of generation of the muskrat liver, clinical medicine meaning (Endoclinology 2004 : Anat.Enbryol. 2004) has been clarified. Furthermore, figures of the nerves innervating the levator palate muscle and mandibular nerve were elucidated (Clin Anat 2004 ; Surg Radiol Anat 2004). At the moment, we make exertions to advancing researches in order to analyze the intimate structures as well as morphogenesis of arteries, veins and nerves coursing in the falciform and teres hepatic ligaments and to clarify the morphological as well as functional relationships-of lymphatic vessel around the xiphoid process with the live in the human cadavers and also in muskrats. These structures and root meanings of these structures are being clarified from the embryological and clinical viewpoints, building up co-operation with clinical researchers. We are also making collaborative researches with doctors in order to open up new method of measurement, diagnosis and treatment of abnormal bones, and also the molecular and histochemical researches of genesis of head and neck muscles. |
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内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 研究課題/領域番号:16590139, 研究期間(年度):2004–2005 | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 出典:「全胚免疫染色と分子生物学的方法による内臓と四肢の神経・血管・筋の形成と再生の解明」研究成果報告書 課題番号16590139 (KAKEN:科学研究費助成事業データベース(国立情報学研究所)) 本文データは著者版報告書より作成 |
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著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://kaken.nii.ac.jp/search/?qm=60004660 | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16590139/ | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-16590139/165901392005kenkyu_seika_hokoku_gaiyo/ |