WEKO3
インデックスリンク
アイテム
{"_buckets": {"deposit": "1c37551c-7cd8-46db-bb11-fbad7da63a89"}, "_deposit": {"created_by": 3, "id": "34890", "owners": [3], "pid": {"revision_id": 0, "type": "depid", "value": "34890"}, "status": "published"}, "_oai": {"id": "oai:kanazawa-u.repo.nii.ac.jp:00034890", "sets": ["2837"]}, "author_link": ["13404", "71374"], "item_9_biblio_info_8": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "1998-03-01", "bibliographicIssueDateType": "Issued"}, "bibliographicPageStart": "51p.", "bibliographicVolumeNumber": "1994-1996", "bibliographic_titles": [{"bibliographic_title": "平成8(1996)年 科学研究費補助金 基盤研究(A) 研究成果報告書"}, {"bibliographic_title": "1996 Fiscal Year Final Research Report", "bibliographic_titleLang": "en"}]}]}, "item_9_creator_33": {"attribute_name": "著者別表示", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Ando, Toshio"}], "nameIdentifiers": [{"nameIdentifier": "71374", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "50184320", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=50184320"}]}]}, "item_9_description_21": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "(a) We manufactured an atomic force microscope that was able to be scanned faster and less susceptible to drift than ordinary AFM apparatuses. This AFM was attached to an epiluminescence fluorescence microscope. To show the high performance of our AFM apparatus we tried to obtain high resolution images of myosin heads (subfragment-1) in aqueous solution. The images revealed fine structures that was comparable to those in an atomic model of S1 derived from x-ray crystallography of S1. In the AFM images we were able to see the ATP binding site as well as the large cleft at the tip of S1. These results proved that our AFM apparatus was on the level of the greatest in the world. (b) We performed a study preparatory to the development of a high-speed AFM in which one image would be acquired within 30msec. We designed and manufactured a high-speed scanner and attained high-speed imaging where one image was obtained within 150msec. By way of experiment we produced an AFM that employed a displacemant detection system suitable for high-speed imaging. We also examinds methods for preparing cantilevers that were suitable for a high-speed AFM.From these preparative studies we were able to secure a scaffolding for the development of the video-rate AFM. (c) To measure bimolecular interaction force at a truly single molecular level we developed a method to capture a single molecule of protein at the apex of a cantilever tip. Using this method we succeeded in quantifying the force field exerted between a single molecule of heavy meromyosin (HMM) and actin. Using this new technology we also discovered that ATP binding to HMM heads induced vibratile structural changes in HMM heads.", "subitem_description_type": "Abstract"}, {"subitem_description": "1.従来のAFM装置より高速に走査でき、ドリフトの影響を受けにくいAFMを製作し、これを蛍光顕微鏡に組み込んだ。この装置の高い性能を示すために、液中にあるミオシン頭部(S1)の像を高い分解能で得ることを試みた。X線結晶解析で得られているS1の原子モデルと細かい点まで比較できるほどの解像度の高い像を得ることができた。ATP結合部位や頭部先端にあるクレフトまで観察することができ、世界最高レベルのAFMであることを実証した。\n2.将来AFMをビデオレートにまで高速化するための準備研究を行った。高速走査できるスキャナーを設計・製作し、1画像を150msecで撮れる走査速度を実現した。高速AFMに適した変位検出法を採用したAFMを試作した。また、高速走査に適したカンチレバ-作製法の検討を行った。これらの研究により最終的な高速化を図るための足がかりを掴むことができた。\n3.真に1分子レベルで分子間相互作用を計測するために、カンチレバ-探針先端に蛋白質1分子を捕捉する方法を開発した。これによって、ミオシンのサブフラグメント(HMM)1分子とアクチン間に働く力の場の諸性質を定量化することに成功した。更に、HMMがATPに結合したあとに振動する構造変化がHMM頭部に起こることを発見した。", "subitem_description_type": "Abstract"}]}, "item_9_description_22": {"attribute_name": "内容記述", "attribute_value_mlt": [{"subitem_description": "研究課題/領域番号:06558099, 研究期間(年度):1994–1996", "subitem_description_type": "Other"}, {"subitem_description": "出典:「生物研究用走査型プローブ顕微鏡の製作」研究成果報告書 課題番号06558099\n (KAKEN:科学研究費助成事業データベース(国立情報学研究所))\n 本文データは著者版報告書より作成", "subitem_description_type": "Other"}]}, "item_9_publisher_17": {"attribute_name": "公開者", "attribute_value_mlt": [{"subitem_publisher": "金沢大学理学部"}]}, "item_9_relation_28": {"attribute_name": "関連URI", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "https://kaken.nii.ac.jp/search/?qm=50184320", "subitem_relation_type_select": "URI"}}, {"subitem_relation_type_id": {"subitem_relation_type_id_text": "https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06558099/", "subitem_relation_type_select": "URI"}}, {"subitem_relation_type_id": {"subitem_relation_type_id_text": "https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-06558099/065580991996kenkyu_seika_hokoku_gaiyo/", "subitem_relation_type_select": "URI"}}]}, "item_9_version_type_25": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_ab4af688f83e57aa", "subitem_version_type": "AM"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "安藤, 敏夫"}], "nameIdentifiers": [{"nameIdentifier": "13404", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "50184320", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=50184320"}, {"nameIdentifier": "50184320", "nameIdentifierScheme": "金沢大学研究者情報", "nameIdentifierURI": "http://ridb.kanazawa-u.ac.jp/public/detail.php?kaken=50184320"}, {"nameIdentifier": "50184320", "nameIdentifierScheme": "研究者番号", "nameIdentifierURI": "https://nrid.nii.ac.jp/nrid/1000050184320"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2017-10-05"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "SC-PR-ANDO-T-kaken 1998-51p.pdf", "filesize": [{"value": "4.9 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_11", "mimetype": "application/pdf", "size": 4900000.0, "url": {"label": "SC-PR-ANDO-T-kaken 1998-51p.pdf", "url": "https://kanazawa-u.repo.nii.ac.jp/record/34890/files/SC-PR-ANDO-T-kaken 1998-51p.pdf"}, "version_id": "34f9cd00-4bd8-4008-bc54-79d70d0a2ff8"}, {"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2017-10-05"}], "displaytype": "detail", "download_preview_message": "", "file_order": 1, "filename": "SC-PR-ANDO-T-kaken 1998-appendix.pdf", "filesize": [{"value": "1.6 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_11", "mimetype": "application/pdf", "size": 1600000.0, "url": {"label": "SC-PR-ANDO-T-kaken 1998-appendix.pdf", "url": "https://kanazawa-u.repo.nii.ac.jp/record/34890/files/SC-PR-ANDO-T-kaken 1998-appendix.pdf"}, "version_id": "eb256f73-6457-4311-880b-c260fc0b31e3"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "原子間力顕微鏡", "subitem_subject_scheme": "Other"}, {"subitem_subject": "AFM", "subitem_subject_scheme": "Other"}, {"subitem_subject": "高速スキャナー", "subitem_subject_scheme": "Other"}, {"subitem_subject": "高速走査", "subitem_subject_scheme": "Other"}, {"subitem_subject": "タンパク質", "subitem_subject_scheme": "Other"}, {"subitem_subject": "分子間相互作用", "subitem_subject_scheme": "Other"}, {"subitem_subject": "ミオシン頭部", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Atomic Force Microscope", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "AFM", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "High-speed Scanner", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "High-speed Scanning", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Protein", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Bi-molecular Interaction", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Myosin Heads", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "jpn"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "research report", "resourceuri": "http://purl.org/coar/resource_type/c_18ws"}]}, "item_title": "生物研究用走査型プローブ顕微鏡の製作", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "生物研究用走査型プローブ顕微鏡の製作"}, {"subitem_title": "Manufacture of Scanning Force Microscope for Biological Research", "subitem_title_language": "en"}]}, "item_type_id": "9", "owner": "3", "path": ["2837"], "permalink_uri": "http://hdl.handle.net/2297/46865", "pubdate": {"attribute_name": "公開日", "attribute_value": "2017-10-05"}, "publish_date": "2017-10-05", "publish_status": "0", "recid": "34890", "relation": {}, "relation_version_is_last": true, "title": ["生物研究用走査型プローブ顕微鏡の製作"], "weko_shared_id": 3}
生物研究用走査型プローブ顕微鏡の製作
http://hdl.handle.net/2297/46865
http://hdl.handle.net/2297/468654a207871-f5f0-45cc-ab53-a00c2bc90427
名前 / ファイル | ライセンス | アクション |
---|---|---|
SC-PR-ANDO-T-kaken 1998-51p.pdf (4.9 MB)
|
||
SC-PR-ANDO-T-kaken 1998-appendix.pdf (1.6 MB)
|
Item type | 報告書 / Research Paper(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2017-10-05 | |||||
タイトル | ||||||
タイトル | 生物研究用走査型プローブ顕微鏡の製作 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Manufacture of Scanning Force Microscope for Biological Research | |||||
言語 | ||||||
言語 | jpn | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_18ws | |||||
資源タイプ | research report | |||||
著者 |
安藤, 敏夫
× 安藤, 敏夫 |
|||||
著者別表示 |
Ando, Toshio
× Ando, Toshio |
|||||
書誌情報 |
平成8(1996)年 科学研究費補助金 基盤研究(A) 研究成果報告書 en : 1996 Fiscal Year Final Research Report 巻 1994-1996, p. 51p., 発行日 1998-03-01 |
|||||
出版者 | ||||||
出版者 | 金沢大学理学部 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | (a) We manufactured an atomic force microscope that was able to be scanned faster and less susceptible to drift than ordinary AFM apparatuses. This AFM was attached to an epiluminescence fluorescence microscope. To show the high performance of our AFM apparatus we tried to obtain high resolution images of myosin heads (subfragment-1) in aqueous solution. The images revealed fine structures that was comparable to those in an atomic model of S1 derived from x-ray crystallography of S1. In the AFM images we were able to see the ATP binding site as well as the large cleft at the tip of S1. These results proved that our AFM apparatus was on the level of the greatest in the world. (b) We performed a study preparatory to the development of a high-speed AFM in which one image would be acquired within 30msec. We designed and manufactured a high-speed scanner and attained high-speed imaging where one image was obtained within 150msec. By way of experiment we produced an AFM that employed a displacemant detection system suitable for high-speed imaging. We also examinds methods for preparing cantilevers that were suitable for a high-speed AFM.From these preparative studies we were able to secure a scaffolding for the development of the video-rate AFM. (c) To measure bimolecular interaction force at a truly single molecular level we developed a method to capture a single molecule of protein at the apex of a cantilever tip. Using this method we succeeded in quantifying the force field exerted between a single molecule of heavy meromyosin (HMM) and actin. Using this new technology we also discovered that ATP binding to HMM heads induced vibratile structural changes in HMM heads. | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | 1.従来のAFM装置より高速に走査でき、ドリフトの影響を受けにくいAFMを製作し、これを蛍光顕微鏡に組み込んだ。この装置の高い性能を示すために、液中にあるミオシン頭部(S1)の像を高い分解能で得ることを試みた。X線結晶解析で得られているS1の原子モデルと細かい点まで比較できるほどの解像度の高い像を得ることができた。ATP結合部位や頭部先端にあるクレフトまで観察することができ、世界最高レベルのAFMであることを実証した。 2.将来AFMをビデオレートにまで高速化するための準備研究を行った。高速走査できるスキャナーを設計・製作し、1画像を150msecで撮れる走査速度を実現した。高速AFMに適した変位検出法を採用したAFMを試作した。また、高速走査に適したカンチレバ-作製法の検討を行った。これらの研究により最終的な高速化を図るための足がかりを掴むことができた。 3.真に1分子レベルで分子間相互作用を計測するために、カンチレバ-探針先端に蛋白質1分子を捕捉する方法を開発した。これによって、ミオシンのサブフラグメント(HMM)1分子とアクチン間に働く力の場の諸性質を定量化することに成功した。更に、HMMがATPに結合したあとに振動する構造変化がHMM頭部に起こることを発見した。 |
|||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 研究課題/領域番号:06558099, 研究期間(年度):1994–1996 | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 出典:「生物研究用走査型プローブ顕微鏡の製作」研究成果報告書 課題番号06558099 (KAKEN:科学研究費助成事業データベース(国立情報学研究所)) 本文データは著者版報告書より作成 |
|||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://kaken.nii.ac.jp/search/?qm=50184320 | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06558099/ | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-06558099/065580991996kenkyu_seika_hokoku_gaiyo/ |