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V-ATPase 阻害剤による分化・細胞死誘導機構
https://doi.org/10.24517/00050626
https://doi.org/10.24517/00050626fbde9f5b-a07d-4686-a702-faf31d919670
名前 / ファイル | ライセンス | アクション |
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PH-PR-OHKUMA-S-kaken 1999-6p.pdf (284.9 kB)
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Item type | 報告書 / Research Paper(1) | |||||
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公開日 | 2018-04-26 | |||||
タイトル | ||||||
タイトル | V-ATPase 阻害剤による分化・細胞死誘導機構 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Mechanism of induction of cell differentiation and cell death by inhibitors against V-ATPase | |||||
言語 | ||||||
言語 | jpn | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_18ws | |||||
資源タイプ | research report | |||||
ID登録 | ||||||
ID登録 | 10.24517/00050626 | |||||
ID登録タイプ | JaLC | |||||
著者別表示 |
Ohkuma, Shoji
× Ohkuma, Shoji |
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書誌情報 |
平成10(1998)年度 科学研究費補助金 基盤研究(C) 研究成果報告書 en : 1998 Fiscal Year Final Research Report 巻 1997-1998, p. 6p., 発行日 1999-03 |
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出版者 | ||||||
出版者 | 金沢大学薬学部 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | 我々は、細胞の増殖・分化・アポトーシスに対する空胞系酸性顆粒の寄与を,bafilomycin A_1の他、プロジギオシン類やデストラキシン等の空胞系プロトンポンプ阻害剤を用いて明らかにするとともに、これら阻害剤のプロトン輸送阻害機構の解明を目指した。 その結果、(1)デストラキシンBとEは、ともにリソソームのプロトンポンプを阻害し、リソソーム内pHを上昇させたが、PC12細胞の分化誘導作用はデストラキシンEにのみ認められた。(2)プロジギオシン類は、そのH^+/Cl共輸送活性によりプロトンポンプを脱共役する、新規H^+/Cl symporterであることを初めて明らかにすることが出来た。また、(3)bafilomycin A_1同様、ブロジギオシン類も神経突起伸展(NOG)を誘導することを発見した。(4)そこで、両プロトンポンプ阻害剤によるNOG誘導作用の特徴を検討したところ、ともに新たなRNA及び蛋白質合成を必要とし、MAPキナーゼ、セリン・スレオニンホスファターゼ、チロシンキナーゼ、チロシンホスファターゼ、カルモジュリン、ホスフォリパーゼA_2依存性であるが、trkチロシンキナーゼやAキナーゼには非依存性である等、両情報伝達経路は酷似していることが判明した。(5)また、tambjamineグループのBE-18591もH^+/Cl symporter活性を示し、免疫抑制や、NOG・アボトーシス誘導作用を示すことを見い出した。(6)ところが、同じH^+/Cl symporterでも、tetrapyrroleは増殖阻害・細胞死誘導作用はあるがNOG作用は示さなかった。 以上の結果より、pH変化やプロトンポンプ活性自身は、少なくともNOG誘導の直接の引き金にはなっていないと結論した。 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | In this research project, we have studied the machanism of (1) inhibition of cell growth, (2) induction of cell differentiation, and (3) induction of apoptosis, by V-ATPase inhibitors like bafilomycins, prodigiosins and destruxins, as well as the mechanims of inhibition of proton translocation by these inhibitors. We found (1) that both destrauxin-B and -E inhibited lysosomal proton pump, but only destruxin-E induced induced neurite out growth (NOG) of PC12 cells., (2) that prodigisins uncoupled various proton pump activities due to their H ^+ /Cl symport activity, (3) that prodigiosins, like bafilomycin A ^<1'> induced neurite out growth (NOG), and (4) that the prodigiosin-induced NOG, like bafolomycin-induced one, required de novo synthesis of new messenger RNA as well as protein synthesis, was sensitive to the inhibitors of MAP kinases, serine/threonine phosphatases, tyr-kinases, tyr-phosphatases, calmidulin and phospholipase A ^<2' > but resistant to inhibitors of trk tyrosine kinase and protein kinase A.BE-18591, a tambjamine group antibiotics, also behaved as H ^+ /CU symporters and inhibited hog gastric proton pump, inhibit *cid secretion by gastric parietal cells, inhibited osteoclast differentiation, suppressed proliferation of immune lymphocytes, and induced both NOG and apoptosis, suggesting that the variety of biological activities displayed by these H ^+ /C1 symporters may be due to their H ^+ /C1 symport activity. However, tetrapyrrole, another H ^+ /CV symporter, did not induced NOG. From these results, we conclud that the effect of V-ATPase inhibitors on the intracellular pH does not participate in the variety of biological activities (including induction of NOG) displayed by these compounds. |
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内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 研究課題/領域番号:09672220, 研究期間(年度):1997-1998 | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 出典:「V-ATPase 阻害剤による分化・細胞死誘導機構」研究成果報告書 課題番号09672220 (KAKEN:科学研究費助成事業データベース(国立情報学研究所)) 本文データは著者版報告書より作成 |
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著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://kaken.nii.ac.jp/search/?qm=10119563 | |||||
関連名称 | https://kaken.nii.ac.jp/search/?qm=10119563 | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09672220/ | |||||
関連名称 | https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09672220/ | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-09672220/096722201998kenkyu_seika_hokoku_gaiyo/ | |||||
関連名称 | https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-09672220/096722201998kenkyu_seika_hokoku_gaiyo/ |