WEKO3
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KOマウスを用いたクラスII型PI3キナーゼC2・の血管形成における役割の解明
https://doi.org/10.24517/00050992
https://doi.org/10.24517/0005099210dfb522-8d5e-4fe4-9e4b-c2474ff00bfd
名前 / ファイル | ライセンス | アクション |
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ME-PR-YOSHIOKA-K-kaken 2011-6p.pdf (323.8 kB)
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Item type | 報告書 / Research Paper(1) | |||||
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公開日 | 2018-06-07 | |||||
タイトル | ||||||
タイトル | KOマウスを用いたクラスII型PI3キナーゼC2・の血管形成における役割の解明 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Mechanistic analysis of blood vessel formation in class II PI3 kinase C2・ Knockout mouse | |||||
言語 | ||||||
言語 | jpn | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_18ws | |||||
資源タイプ | research report | |||||
ID登録 | ||||||
ID登録 | 10.24517/00050992 | |||||
ID登録タイプ | JaLC | |||||
著者 |
吉岡, 和晃
× 吉岡, 和晃 |
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著者別表示 |
Yoshioka, Kazuaki
× Yoshioka, Kazuaki |
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書誌情報 |
平成22(2010)年度 科学研究費補助金 基盤研究(C) 研究成果報告書 en : 2010 Fiscal Year Final Research Report 巻 2008-2010, p. 6p., 発行日 2011-05-19 |
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出版者 | ||||||
出版者 | 金沢大学医薬保健研究域医学系 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | 本研究では、"クラスII型PI3キナーゼC2α"の機能、特に血管恒常性における役割を明らかにするために、C2α遺伝子KOマウスを用いてC2αの生理機能と血管障害における役割を解析することを目的として実施した。KOマウスの表現型解析から、胎生期血管形成プロセスの異常は血管新生、特に血管成熟過程に起因する可能性が高いと考えられた。また、C2αヘテロKOマウス血管障害モデルでは、解離性大動脈瘤が高頻度に観察された。C2αはこれまで全く生理機能が知られてなく、クラスI型PI3キナーゼとは異なる基質特異性、細胞内分布を示すことから、血管恒常性維持に必須の新たな機能的役割を持つ細胞内制御因子でありことが明らかとなった。 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Phosphatidylinositol-3-OH kinase (PI3K) family, which comprises three classes, regulates a diverse array of cellular processes through the generation of 3-phosphoinositides. Although class I PI3Ks including p110α, p110β, p110δ and p110γ isoforms were well characterized among three classes, the in vivo physiological functions of class II PI3Ks, which comprise three members PI3K-C2α (C2α), C2β and C2γ, remain largely unknown. We found that Pik3c2a gene-global disruption (C2α KO) and conditional loss of C2α in endothelial cells (ECs) in mice, but not in vascular smooth muscles (VSMCs) and cardiomyocytes, caused embryonic lethality due to impairment of developmental angiogenesis characterized by incomplete EC sprouting and mural-cell (VSMCs and pericytes) coverage. Finally, C2α-haploinsufficient mice are alive, but exhibit vascular hyperpermeability and a higher incidence of dissecting aortic aneurysms with rupture on angiotensin-II (AngII) infusion. These results provide the first evidence that C2α plays a novel essential role in endothelial physiology, particularly angiogenesis and barrier integrity, through regulating endosomal trafficking and underscore broader roles for PI3K family members in vascular physiology. | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 研究課題/領域番号:20590207, 研究期間(年度):2008-2010 | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 出典:研究課題「KOマウスを用いたクラスII型PI3キナーゼC2・の血管形成における役割の解明」課題番号20590207 (KAKEN:科学研究費助成事業データベース(国立情報学研究所)) (https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-20590207/20590207seika/)を加工して作成 |
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著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://kaken.nii.ac.jp/search/?qm=80333368 | |||||
関連名称 | https://kaken.nii.ac.jp/search/?qm=80333368 | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20590207/ | |||||
関連名称 | https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20590207/ | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-20590207/20590207seika/ | |||||
関連名称 | https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-20590207/20590207seika/ |