WEKO3
インデックスリンク
アイテム
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Expression of KRTA5 and B5 was approx. 3.5 times as much and p-actin expression in normal hair, whereas that in alopecia hair was approx. 5.5 times. KRT15 expression in normal hair and alopecia hair was respectively approx. 0.5 times and 6.5 times as that of β-actin, viz. KRT expression in alopecia was more than 13 times higher than that in normal hair. TYR and TYRP1 expression was approx. 0.1 and 0.3 times as that of (-actin, respectively. It is not always observed in all alopecia hair but some alopecia hair showed almost null expression of them. Even in alopecia patient, hair obtained from the non-bald area displayed the expression pattern similar with normal hair. 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毛髪の形成に関わる遺伝子・蛋白の同定と脱毛抑制・促進因子の解析
https://doi.org/10.24517/00051088
https://doi.org/10.24517/00051088dba28647-dfa7-4c7e-bd00-5dee3ddca0b0
名前 / ファイル | ライセンス | アクション |
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ME-PR-SAIJYO-K-kaken 2008-6p.pdf (287.4 kB)
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Item type | 報告書 / Research Paper(1) | |||||
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公開日 | 2018-06-18 | |||||
タイトル | ||||||
タイトル | 毛髪の形成に関わる遺伝子・蛋白の同定と脱毛抑制・促進因子の解析 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Identification of genes and proteins related to hair growth and analysis of alopecia inducing and preventing factor | |||||
言語 | ||||||
言語 | jpn | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_18ws | |||||
資源タイプ | research report | |||||
ID登録 | ||||||
ID登録 | 10.24517/00051088 | |||||
ID登録タイプ | JaLC | |||||
著者別表示 |
Saijoh, Kiyoshi
× Saijoh, Kiyoshi |
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書誌情報 |
平成19(2007)年度 科学研究費補助金 基盤研究(C) 研究成果報告書 en : 2007 Fiscal Year Final Research Report 巻 2006-2007, p. 6p., 発行日 2008-03 |
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出版者 | ||||||
出版者 | 金沢大学医薬保健研究域医学系 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | 男性正常頭髪及び男性型禿頭進行部位頭髪毛根と外毛根鞘からmRNAを直接抽出することを可能にした。得られたmRNAの発現パターンの異同を明らかにするためにDNAチップによる解析を行った。まず,毛髪の質を決定すると考えられるケラチンの分子種と両頭髪でしばしば観察される色調の違いに関わると考えられるメラニン形成に関わる遺伝子に注目し,分析を行った。その結果正常毛では,毛髪ケラチンKA36,ケラチン25などが高発現するのに対し,禿頭毛では毛髪ケラチン酸性5(KRTHA5)と塩基性5(KRTHB5)及びケラチン15(KRT15)が高発現しており,メラニン形成カスケードの律速酵素であるタイロシネース(TYR)とその補助蛋白であるタイロシネース関連蛋白1(TYRP1)の低発現が認められた。この発現量の差をRT-PCRを用い定量したところ,KRTHA5とB5の正常毛での発現量はβ-アクチン発現量の約3.5倍であるのに対し,禿頭毛では約5.5倍であった。KRT15の正常毛での発現量はβ-アクチン発現量の約0.5倍であるのに対し,禿頭毛では約6.5倍であり,相対発現量としては13倍以上であった。TYRとTYRP1は,減少しない禿頭毛もあるものの,正常毛でのそれぞれβ-アクチンの約0.1倍と約0.3倍の発現が激減する症例のあることが明かとなった。また,禿頭者毛髪でも,禿頭進行部以外では正常毛と同じパターンの発現を示すことも明かとなった。そこで,このケラチンの発現パターンにより,禿頭がどこまで進行するのか判定できると考え,特許申請した。 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | At first, we succeed to purify mRNA directly from hair root and outer root sheath. Using mRNA from the normal male scalp hair and the scalp hair from male-type alopecia, expression difference among mRNA species were analyzed by DNA tip analysis. We focused on difference of expression of keratin that is supposed to affect the quality of hair. We also focused on genes related to melanin synthesis like tyrosinase (TYR) and tyrosinase related protein 1 (TYRP1) because hair color changes were frequently observed in male-type alopecia. TYR is a rate-limiting enzyme of this cascade and TYRP1 is essential to maintain TYR function. On DNA tip analysis, hair keratin KA36 and keratin 25 showed abundant expression in normal hair. In alopecia pair, hair keratin acidic 5 (KRTA5) and basic 5 (KRTB5) as well as keratin 15 (KRT15) were abundantly expressed but TYR and TYRP I were not. Next, we tried to quantitate their expression using RT-PCR. Expression of KRTA5 and B5 was approx. 3.5 times as much and p-actin expression in normal hair, whereas that in alopecia hair was approx. 5.5 times. KRT15 expression in normal hair and alopecia hair was respectively approx. 0.5 times and 6.5 times as that of β-actin, viz. KRT expression in alopecia was more than 13 times higher than that in normal hair. TYR and TYRP1 expression was approx. 0.1 and 0.3 times as that of (-actin, respectively. It is not always observed in all alopecia hair but some alopecia hair showed almost null expression of them. Even in alopecia patient, hair obtained from the non-bald area displayed the expression pattern similar with normal hair. Thus, we consider that the analysis of these genes can show us the way to distinguish the area affected and non-affected by male-type alopecia and apply to the patent. | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 研究課題/領域番号:18590551, 研究期間(年度):2006-2007 | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 出典:「毛髪の形成に関わる遺伝子・蛋白の同定と脱毛抑制・促進因子の解析」研究成果報告書 課題番号18590551 (KAKEN:科学研究費助成事業データベース(国立情報学研究所)) 本文データは著者版報告書より作成 |
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著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://kaken.nii.ac.jp/search/?qm=00178469 | |||||
関連名称 | https://kaken.nii.ac.jp/search/?qm=00178469 | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18590551/ | |||||
関連名称 | https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18590551/ | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-18590551/185905512007kenkyu_seika_hokoku_gaiyo/ | |||||
関連名称 | https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-18590551/185905512007kenkyu_seika_hokoku_gaiyo/ |