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新生児・乳児期における好塩基球表面IgEならびにCD38抗原発現とその機能的意義
https://doi.org/10.24517/00051178
https://doi.org/10.24517/00051178d076d712-0114-49a1-b9a2-26666e9e2352
名前 / ファイル | ライセンス | アクション |
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HO-PR-YACHIE-A-kaken 1997-8p.pdf (436.7 kB)
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Item type | 報告書 / Research Paper(1) | |||||
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公開日 | 2018-06-22 | |||||
タイトル | ||||||
タイトル | 新生児・乳児期における好塩基球表面IgEならびにCD38抗原発現とその機能的意義 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | IgE binding and CD38 expression on basophil surface during infancy and their functional relevance | |||||
言語 | ||||||
言語 | jpn | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_18ws | |||||
資源タイプ | research report | |||||
ID登録 | ||||||
ID登録 | 10.24517/00051178 | |||||
ID登録タイプ | JaLC | |||||
著者 |
谷内江, 昭宏
× 谷内江, 昭宏 |
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著者別表示 |
Yachie, Akihiro
× Yachie, Akihiro |
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書誌情報 |
平成8(1996)年度 科学研究費補助金 基盤研究(C) 研究成果報告書 en : 1996 Fiscal Year Final Research Report 巻 1995-1996, p. 8p., 発行日 1997-03 |
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出版者 | ||||||
出版者 | 金沢大学医薬保健研究域医学系 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | 末梢血好塩基球表面にはFcεRIが強く発現されている。しかし、その発現調節機構に関しては不明のことが多く、特に小児末梢血好塩基球表面のFcεRI発現に関して検討した報告は見当たらない。本研究では、新生児ならびに種々の年令の小児末梢血好塩基球表面のIgE結合ならびにFcεRI発現をフローサイトメトリーにより評価し、さらにIgEによる発現調節機構を検討した。また血清中IgE濃度を単クローン抗体を用いた高感度ELISA法により定量した。好塩基球表面IgE結合は正常対照に比し、アレルギー疾患群で著明な高値を示し、その差は低年令群においてより顕著に認められた。表面結合IgEは血清IgE濃度とよく相関し、血清IgEが100ng/ml前後で急速に好塩基球表面結合が増加し、300ng/ml付近でほぼプラトーに達した。新生児臍帯血では表面IgE結合を示す細胞はほとんど検出できなかったが、高濃度のIgE存在下で短時間培養することにより急速にIgE結合が観察され、長時間の培養によりさらにIgE結合が増強された。好塩基球のFcεRI発現は表面IgE結合と同様、血清IgE濃度に強く依存した。臍帯血中好塩基球表面のFcεRI発現は極めて低値であったが、高濃度のIgEを添加し培養するとその発現が増強した。このような発現増強は温度ならびに蛋白合成に依存性であり、de novoの蛋白合成を必要とすると考えられた。しかし、臍帯血におけるFcεRIα mRNA発現は構成的に認められ、IgE添加によっても著明な増強は認められないことより、転写レベル以外の調節機構が関与していることが示唆された。 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Peripheral blood basophils express FcepsilonRI on the surface. The regulatory mechanism of its expression is, however, largely unknown. In particular, there has been no report regarding the FcepsilonRI expression on circulating basophils during childhood. In this study, we evaluated IgE binding and FcepsilonRI expression on basophil surface in neonates and in children of various age groups. Furthermore, the regulation of FcepsilonRI by monomeric IgE was examined. In addition, serum IgE concentration was determined by a sensitive ELISA assay using anti-IgE monoclonal antibodies. Basophil-bound IgE was significantly high among allergics than normal controls. The difference was more prominent among younger age groups. The surface-bound IgE correlated very well with serum IgE concentrations. The level increased rapidly around 100 ng/ml of IgE and it reached a plateau when serum IgE was higher than 300 ng/ml. IgE-binding cell was undetectable within neonatal cord blood. However, IgE binding was induced rapidly when the cells were incubated with excess monomeric IgE for short period. Longer incubation resulted in further potentiation of IgE binding. FcepsilonRI expression on basophil surface also correlated well with serum IgE.FcepsilonRI expression on neonatal basophils was relatively low, but the level was upregulated when these cells were incubated with IgE.The enhancement was dependent on de novo protein synthesis. Constitutive synthesis of FcepsilonRI mRNA synthesis was observed in neonatal blood and the level was not significantly increased after the addition of IgE,indicating that a mechanism other than mRNA transcription is involved in the regulation of surface IgE receptor expression. | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 研究課題/領域番号:07670847, 研究期間(年度):1995-1996 | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 出典:「新生児・乳児期における好塩基球表面IgEならびにCD38抗原発現とその機能的意義」研究成果報告書 課題番号07670847 (KAKEN:科学研究費助成事業データベース(国立情報学研究所)) 本文データは著者版報告書より作成 |
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著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://kaken.nii.ac.jp/search/?qm=40210281 | |||||
関連名称 | https://kaken.nii.ac.jp/search/?qm=40210281 | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07670847/ | |||||
関連名称 | https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07670847/ | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-07670847/076708471996kenkyu_seika_hokoku_gaiyo/ | |||||
関連名称 | https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-07670847/076708471996kenkyu_seika_hokoku_gaiyo/ |