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脂肪由来幹細胞を応用した骨軟部腫瘍切除後組織欠損の再生医療と蛍光イメージング評価
https://doi.org/10.24517/00051524
https://doi.org/10.24517/00051524a345e382-1b02-42f4-9c9c-332937bec72c
名前 / ファイル | ライセンス | アクション |
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HO-PR-HAYASHI-K-kaken 2017-5p.pdf (301.5 kB)
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Item type | 報告書 / Research Paper(1) | |||||
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公開日 | 2018-07-05 | |||||
タイトル | ||||||
タイトル | 脂肪由来幹細胞を応用した骨軟部腫瘍切除後組織欠損の再生医療と蛍光イメージング評価 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Regeneration using adipose-derived stem cells and fluorescent imaging evaluation for a defect after bone and soft tissue tumor surgery | |||||
言語 | ||||||
言語 | jpn | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_18ws | |||||
資源タイプ | research report | |||||
ID登録 | ||||||
ID登録 | 10.24517/00051524 | |||||
ID登録タイプ | JaLC | |||||
著者 |
林, 克洋
× 林, 克洋 |
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著者別表示 |
Hayashi, Katsuhiro
× Hayashi, Katsuhiro |
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提供者所属 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 金沢大学附属病院整形外科 | |||||
書誌情報 |
平成28(2016)年度 科学研究費補助金 基盤研究(C) 研究成果報告書 en : 2016 Fiscal Year Final Research Report 巻 2014-04-01 - 2017-03-31, p. 5p., 発行日 2017-05-22 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | 間葉系幹細胞(MSC)においてp53蛋白は骨芽細胞分化や脂肪細胞分化を抑制する。同一個体からALT/WDL組織と正常脂肪組織を採取し、MSCを分離培養し、骨芽細胞分化能と脂肪細胞分化能を比較した。脂肪分化能においては、我々の予想に反して、正常脂肪由来MSCにおいてより多くの脂肪滴が認められた。対照的に、骨芽細胞分化能においては、ALT/WDL由来MSCでより高い分化能を認めた。ALP染色に基づく骨芽細胞分化能の差異はMDM2特異的阻害薬Nutlin-3濃度が上昇するにつれて減少した。ALT/WDL由来MSCにおけるMDM2遺伝子の増幅がより高い骨芽細胞分化能をもたらしている可能性が示唆された。 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | We compared osteogenic and adipogenic differentiation potency between MSCs isolated and cultured from normal adipose tissues and atypical lipomatous tumor/well-differentiated liposarcoma (ALT/WDL) from the same patients. We found greater osteogenic differentiation potency in ALT/WDL-derived MSCs than in normal fat-derived MSCs. In adipogenic differentiation, contrary to our expectations, more fatty acid droplets were observed in normal fatderived MSCs than in ALT/WDL-derived MSCs. In osteogenic differentiation, as expected, MSCs derived from ALT/WDL had higher differentiation potency than normal fat-derived MSCs. The gap of osteogenic differentiation potency based on ALP staining between ALT/WDL-derived MSCs and normal fat-derived MSCs disappeared as Nutlin-3 concentration increased, suggesting that greater osteogenic differentiation could be caused by amplied MDM2 in ALT/WDL. | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 研究課題/領域番号:26462271, 研究期間(年度):2014-04-01 - 2017-03-31 | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 出典:「脂肪由来幹細胞を応用した骨軟部腫瘍切除後組織欠損の再生医療と蛍光イメージング評価」研究成果報告書 課題番号26462271 (KAKEN:科学研究費助成事業データベース(国立情報学研究所)) (https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-26462271/26462271seika/)を加工して作成 |
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著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://kaken.nii.ac.jp/search/?qm=80507054 | |||||
関連名称 | https://kaken.nii.ac.jp/search/?qm=80507054 | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-26462271/ | |||||
関連名称 | https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-26462271/ | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-26462271/26462271seika/ | |||||
関連名称 | https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-26462271/26462271seika/ |