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制御性樹状細胞の大量培養を用いた新たな造血幹細胞移植の開発
https://doi.org/10.24517/00052973
https://doi.org/10.24517/00052973f41c03e8-c9f9-4348-ba04-1e7e058e543a
名前 / ファイル | ライセンス | アクション |
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ME-PR-MAEBA-H-kaken 2011-6p.pdf (596.3 kB)
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Item type | 報告書 / Research Paper(1) | |||||
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公開日 | 2018-12-21 | |||||
タイトル | ||||||
タイトル | 制御性樹状細胞の大量培養を用いた新たな造血幹細胞移植の開発 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | The treatment strategy for GVHD by infusion of PUVA-treated dendritic cells | |||||
言語 | ||||||
言語 | jpn | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_18ws | |||||
資源タイプ | research report | |||||
ID登録 | ||||||
ID登録 | 10.24517/00052973 | |||||
ID登録タイプ | JaLC | |||||
著者 |
前馬, 秀昭
× 前馬, 秀昭 |
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著者別表示 |
Maeba, Hideaki
× Maeba, Hideaki |
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書誌情報 |
平成22(2010)年度 科学研究費補助金 若手研究(B) 研究成果報告書 en : 2010 Fiscal Year Final Research Report 巻 2009-2010, p. 6p., 発行日 2011-05-18 |
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出版者 | ||||||
出版者 | 金沢大学附属病院小児科 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | マウス骨髄細胞からGM-CSFを添加し未熟樹状細胞を大量に培養した後、psolarenと紫外線を併用し(いわゆるPUVA療法の応用)制御性樹状細胞を大量に作製することに成功した。その細胞は、混合リンパ球反応(MLR)においてMHC非拘束性に抑制性の機能を有していた。メカニズムは、抗炎症性サイトカイン(IL-10やTGF-β)によるものではなく、細胞間の接触によって引き起こされていた。トリプトファン代謝による細胞増殖の抑制をみるため、indoleamine 2,3-dioxygenase (IDO)の発現を定量PCR法にて測定したところ、骨髄由来の樹状細胞に比べて5倍以上発現していた。IDOを発現し、MHC非拘束性に抑制機能を有するPUVA処理した樹状細胞による細胞療法は、致死的なGVHDに対して有用な治療法と成りうる可能性がある。 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Bone marrow-derived cultured DCs acquired tolerogenicity by PUVA (psoralen+UVA) treatment in mice. In MLR assays, strong tolerogenicity was observed when adding PUVA-DCs generated from the same strain of stimulator cells, or responder cells, or even from third party strain into MLR mixture. That is, the PUVA-treated DCs have tolerogenic function in a MHC-independent manner, in part, due to the reduced expression levels of CD80 and CD86. To clarify the mechanisms of how PUVA-treated DCs induce tolerogenicity in further detail, we performed MLR with the addition of neutralizing antibodies against IL-10 or TGF-b1 or both. Neutralization of immunosuppressive cytokines had no effects on MLR. We then showed that cell-to-cell contact between PUVA-DCs and alloreactive T-cells was needed to mediate the regulatory effect by transwell experiment. Next we compared the expression of the indoleamine 2,3-dioxygenase (IDO), which induces T-cell anergy by tryptophan depletion and by the production of metabolic byproducts collectively known as kynurenines, by real-time PCR between PUVA-DCs and BM-DCs. An increase IDO gene transcription level was observed in PUVA-DCs about 5 times more than in BM-DCs (p<0.01). In conclusion, PUVA-DCs acquired regulatory function in a MHC-independent manner by upregulation of IDO. Infusion of PUVA-treated DCs could have a great potential to treat lethal acute GVHD in clinical settings. | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 出典:研究課題「制御性樹状細胞の大量培養を用いた新たな造血幹細胞移植の開発」課題番号21790973 (KAKEN:科学研究費助成事業データベース(国立情報学研究所)) (https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-21790973/21790973seika/)を加工して作成 |
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著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://kaken.nii.ac.jp/search/?qm=10419335 | |||||
関連名称 | https://kaken.nii.ac.jp/search/?qm=10419335 | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21790973/ | |||||
関連名称 | https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21790973/ | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-21790973/21790973seika/ | |||||
関連名称 | https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-21790973/21790973seika/ |