WEKO3
インデックスリンク
アイテム
{"_buckets": {"deposit": "2eb3baf4-5285-4809-8134-18b8ab8ccd5d"}, "_deposit": {"created_by": 18, "id": "47559", "owners": [18], "pid": {"revision_id": 0, "type": "depid", "value": "47559"}, "status": "published"}, "_oai": {"id": "oai:kanazawa-u.repo.nii.ac.jp:00047559", "sets": ["1134"]}, "author_link": ["23919", "80335", "23442", "707", "84815", "21404", "174", "84833", "55", "76763"], "item_4_biblio_info_8": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2019-03-01", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "2", "bibliographicPageEnd": "280", "bibliographicPageStart": "263", "bibliographicVolumeNumber": "69", "bibliographic_titles": [{"bibliographic_title": "Journal of Physiological Sciences"}]}]}, "item_4_creator_33": {"attribute_name": "著者別表示", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "吉岡, 和晃"}], "nameIdentifiers": [{"nameIdentifier": "174", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "80333368", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=80333368"}, {"nameIdentifier": "80333368", "nameIdentifierScheme": "金沢大学研究者情報", "nameIdentifierURI": "http://ridb.kanazawa-u.ac.jp/public/detail.php?kaken=80333368"}, {"nameIdentifier": "80333368", "nameIdentifierScheme": "研究者番号", "nameIdentifierURI": "https://nrid.nii.ac.jp/nrid/1000080333368"}]}, {"creatorNames": [{"creatorName": "安藝, 翔"}], "nameIdentifiers": [{"nameIdentifier": "707", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "80767210", "nameIdentifierScheme": "金沢大学研究者情報", "nameIdentifierURI": "http://ridb.kanazawa-u.ac.jp/public/detail.php?kaken=80767210"}, {"nameIdentifier": "80767210", "nameIdentifierScheme": "研究者番号", "nameIdentifierURI": "https://nrid.nii.ac.jp/nrid/1000080767210"}]}, {"creatorNames": [{"creatorName": "多久和, 典子"}], "nameIdentifiers": [{"nameIdentifier": "84815", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "70150290", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=70150290"}]}, {"creatorNames": [{"creatorName": "多久和, 陽"}], "nameIdentifiers": [{"nameIdentifier": "55", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "60171592", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=60171592"}, {"nameIdentifier": "60171592", "nameIdentifierScheme": "金沢大学研究者情報", "nameIdentifierURI": "http://ridb.kanazawa-u.ac.jp/public/detail.php?kaken=60171592"}, {"nameIdentifier": "60171592", "nameIdentifierScheme": "研究者番号", "nameIdentifierURI": "https://nrid.nii.ac.jp/nrid/1000060171592"}]}]}, "item_4_description_21": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Pinocytosis is an important fundamental cellular process that is used by the cell to transport fluid and solutes. Phosphoinositide 3-kinases (PI3Ks) regulate a diverse array of dynamic membrane events. However, it is not well-understood which PI3K isoforms are involved in specific mechanisms of pinocytosis. We performed knockdown studies of endogenous PI3K isoforms and clathrin heavy chain (CHC) mediated by small interfering RNA (siRNA). The results demonstrated that the class II PI3K PI3K-C2α and PI3K-C2β, but not the class I or III PI3K, were required for pinocytosis, based on an evaluation of fluorescein-5-isothiocyanate (FITC)–dextran uptake in endothelial cells. Pinocytosis was partially dependent on both clathrin and dynamin, and both PI3K-C2α and PI3K-C2β were required for clathrin-mediated—but not clathrin-non-mediated—FITC-dextran uptake at the step leading up to its delivery to early endosomes. Both PI3K-C2α and PI3K-C2β were co-localized with clathrin-coated pits and vesicles. However, PI3K-C2β, but not PI3K-C2α, was highly co-localized with actin filament-associated clathrin-coated structures and required for actin filament formation at the clathrin-coated structures. These results indicate that PI3K-C2α and PI3K-C2β play differential, indispensable roles in clathrin-mediated pinocytosis. © 2018, The Physiological Society of Japan and Springer Japan KK, part of Springer Nature.", "subitem_description_type": "Abstract"}]}, "item_4_description_22": {"attribute_name": "内容記述", "attribute_value_mlt": [{"subitem_description": "Embargo Period 12 months", "subitem_description_type": "Other"}]}, "item_4_description_5": {"attribute_name": "提供者所属", "attribute_value_mlt": [{"subitem_description": "金沢大学医薬保健研究域医学系", "subitem_description_type": "Other"}]}, "item_4_identifier_registration": {"attribute_name": "ID登録", "attribute_value_mlt": [{"subitem_identifier_reg_text": "10.24517/00053883", "subitem_identifier_reg_type": "JaLC"}]}, "item_4_publisher_17": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Physiological Society of Japan 日本生理学会 / Springer Nature"}]}, "item_4_relation_12": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type": "isVersionOf", "subitem_relation_type_id": {"subitem_relation_type_id_text": "10.1007/s12576-018-0644-2", "subitem_relation_type_select": "DOI"}}]}, "item_4_relation_28": {"attribute_name": "関連URI", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "https://link.springer.com/article/10.1007%2Fs12576-018-0644-2"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://link.springer.com/article/10.1007%2Fs12576-018-0644-2", "subitem_relation_type_select": "URI"}}]}, "item_4_rights_23": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "copyright © 2018, The Physiological Society of Japan and Springer Japan KK, part of Springer Nature."}]}, "item_4_source_id_11": {"attribute_name": "NCID", "attribute_value_mlt": [{"subitem_source_identifier": "AA12129145", "subitem_source_identifier_type": "NCID"}]}, "item_4_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "1880-6546", "subitem_source_identifier_type": "ISSN"}]}, "item_4_version_type_25": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_ab4af688f83e57aa", "subitem_version_type": "AM"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Aung, Khin Thuzar"}], "nameIdentifiers": [{"nameIdentifier": "84833", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yoshioka, Kazuaki"}], "nameIdentifiers": [{"nameIdentifier": "76763", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "80333368", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=80333368"}]}, {"creatorNames": [{"creatorName": "Aki, Sho"}], "nameIdentifiers": [{"nameIdentifier": "23442", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "80767210", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=80767210"}]}, {"creatorNames": [{"creatorName": "Ishimaru, Kazuhiro"}], "nameIdentifiers": [{"nameIdentifier": "80335", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "70595446", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=70595446"}]}, {"creatorNames": [{"creatorName": "Takuwa, Noriko"}], "nameIdentifiers": [{"nameIdentifier": "21404", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "70150290", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=70150290"}, {"nameIdentifier": "70150290", "nameIdentifierScheme": "研究者番号", "nameIdentifierURI": "https://nrid.nii.ac.jp/nrid/1000070150290"}]}, {"creatorNames": [{"creatorName": "Takuwa, Yoh"}], "nameIdentifiers": [{"nameIdentifier": "23919", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "60171592", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=60171592"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2020-03-02"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "ME-PR-TAKUWA-Y-263.pdf", "filesize": [{"value": "10.1 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_11", "mimetype": "application/pdf", "size": 10100000.0, "url": {"label": "ME-PR-TAKUWA-Y-263.pdf", "url": "https://kanazawa-u.repo.nii.ac.jp/record/47559/files/ME-PR-TAKUWA-Y-263.pdf"}, "version_id": "3d4d2fa5-4fb2-4464-aa0a-950e5db1b2e8"}, {"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2020-03-02"}], "displaytype": "detail", "download_preview_message": "", "file_order": 1, "filename": "ME-PR-TAKUWA-Y-263-supplementary video 1.mp4", "filesize": [{"value": "2.5 MB"}], "format": "video/mp4", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_11", "mimetype": "video/mp4", "size": 2500000.0, "url": {"label": "ME-PR-TAKUWA-Y-263-supplementary video 1.mp4", "url": "https://kanazawa-u.repo.nii.ac.jp/record/47559/files/ME-PR-TAKUWA-Y-263-supplementary video 1.mp4"}, "version_id": "abe256e5-606f-4847-a9b0-fac542c2741c"}, {"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2020-03-02"}], "displaytype": "detail", "download_preview_message": "", "file_order": 2, "filename": "ME-PR-TAKUWA-Y-263-supplementary video 2.mp4", "filesize": [{"value": "2.2 MB"}], "format": "video/mp4", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_11", "mimetype": "video/mp4", "size": 2200000.0, "url": {"label": "ME-PR-TAKUWA-Y-263-supplementary video 2.mp4", "url": "https://kanazawa-u.repo.nii.ac.jp/record/47559/files/ME-PR-TAKUWA-Y-263-supplementary video 2.mp4"}, "version_id": "0550d170-9bcc-497c-851a-d6a6ca56d8f3"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "Class II PI3K", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Clathrin", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Endothelial cell", "subitem_subject_scheme": "Other"}, {"subitem_subject": "PI3K-C2α", "subitem_subject_scheme": "Other"}, {"subitem_subject": "PI3K-C2β", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Pinocytosis", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "The class II phosphoinositide 3-kinases PI3K-C2α and PI3K-C2β differentially regulate clathrin-dependent pinocytosis in human vascular endothelial cells", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "The class II phosphoinositide 3-kinases PI3K-C2α and PI3K-C2β differentially regulate clathrin-dependent pinocytosis in human vascular endothelial cells"}]}, "item_type_id": "4", "owner": "18", "path": ["1134"], "permalink_uri": "https://doi.org/10.24517/00053883", "pubdate": {"attribute_name": "公開日", "attribute_value": "2019-04-25"}, "publish_date": "2019-04-25", "publish_status": "0", "recid": "47559", "relation": {}, "relation_version_is_last": true, "title": ["The class II phosphoinositide 3-kinases PI3K-C2α and PI3K-C2β differentially regulate clathrin-dependent pinocytosis in human vascular endothelial cells"], "weko_shared_id": -1}
The class II phosphoinositide 3-kinases PI3K-C2α and PI3K-C2β differentially regulate clathrin-dependent pinocytosis in human vascular endothelial cells
https://doi.org/10.24517/00053883
https://doi.org/10.24517/000538830f4e3de1-e3ba-4fdc-8bb9-b788b5163737
名前 / ファイル | ライセンス | アクション |
---|---|---|
ME-PR-TAKUWA-Y-263.pdf (10.1 MB)
|
||
ME-PR-TAKUWA-Y-263-supplementary video 1.mp4 (2.5 MB)
|
||
ME-PR-TAKUWA-Y-263-supplementary video 2.mp4 (2.2 MB)
|
Item type | 学術雑誌論文 / Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2019-04-25 | |||||
タイトル | ||||||
タイトル | The class II phosphoinositide 3-kinases PI3K-C2α and PI3K-C2β differentially regulate clathrin-dependent pinocytosis in human vascular endothelial cells | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
ID登録 | ||||||
ID登録 | 10.24517/00053883 | |||||
ID登録タイプ | JaLC | |||||
著者 |
Aung, Khin Thuzar
× Aung, Khin Thuzar× Yoshioka, Kazuaki× Aki, Sho× Ishimaru, Kazuhiro× Takuwa, Noriko× Takuwa, Yoh |
|||||
著者別表示 |
吉岡, 和晃
× 吉岡, 和晃× 安藝, 翔× 多久和, 典子× 多久和, 陽 |
|||||
提供者所属 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 金沢大学医薬保健研究域医学系 | |||||
書誌情報 |
Journal of Physiological Sciences 巻 69, 号 2, p. 263-280, 発行日 2019-03-01 |
|||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1880-6546 | |||||
NCID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA12129145 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1007/s12576-018-0644-2 | |||||
出版者 | ||||||
出版者 | Physiological Society of Japan 日本生理学会 / Springer Nature | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Pinocytosis is an important fundamental cellular process that is used by the cell to transport fluid and solutes. Phosphoinositide 3-kinases (PI3Ks) regulate a diverse array of dynamic membrane events. However, it is not well-understood which PI3K isoforms are involved in specific mechanisms of pinocytosis. We performed knockdown studies of endogenous PI3K isoforms and clathrin heavy chain (CHC) mediated by small interfering RNA (siRNA). The results demonstrated that the class II PI3K PI3K-C2α and PI3K-C2β, but not the class I or III PI3K, were required for pinocytosis, based on an evaluation of fluorescein-5-isothiocyanate (FITC)–dextran uptake in endothelial cells. Pinocytosis was partially dependent on both clathrin and dynamin, and both PI3K-C2α and PI3K-C2β were required for clathrin-mediated—but not clathrin-non-mediated—FITC-dextran uptake at the step leading up to its delivery to early endosomes. Both PI3K-C2α and PI3K-C2β were co-localized with clathrin-coated pits and vesicles. However, PI3K-C2β, but not PI3K-C2α, was highly co-localized with actin filament-associated clathrin-coated structures and required for actin filament formation at the clathrin-coated structures. These results indicate that PI3K-C2α and PI3K-C2β play differential, indispensable roles in clathrin-mediated pinocytosis. © 2018, The Physiological Society of Japan and Springer Japan KK, part of Springer Nature. | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Embargo Period 12 months | |||||
権利 | ||||||
権利情報 | copyright © 2018, The Physiological Society of Japan and Springer Japan KK, part of Springer Nature. | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://link.springer.com/article/10.1007%2Fs12576-018-0644-2 | |||||
関連名称 | https://link.springer.com/article/10.1007%2Fs12576-018-0644-2 |