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Fas誘導性アポトーシス細胞死における活性酸素の意義
https://doi.org/10.24517/00054993
https://doi.org/10.24517/000549930058f1f4-325f-47f6-9912-f70731d8c2b4
名前 / ファイル | ライセンス | アクション |
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ME-PR-KASAHARA-Y-kaken 1998-12p.pdf (466.7 kB)
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Item type | 報告書 / Research Paper(1) | |||||
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公開日 | 2019-07-26 | |||||
タイトル | ||||||
タイトル | Fas誘導性アポトーシス細胞死における活性酸素の意義 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Significance of Reactive Oxygen Intermediates in Fas-mediated apoptosis | |||||
言語 | ||||||
言語 | jpn | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_18ws | |||||
資源タイプ | research report | |||||
ID登録 | ||||||
ID登録 | 10.24517/00054993 | |||||
ID登録タイプ | JaLC | |||||
著者 |
笠原, 善仁
× 笠原, 善仁 |
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著者別表示 |
Kasahara, Yoshihito
× Kasahara, Yoshihito |
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書誌情報 |
平成9(1997)年度 科学研究費補助金 基盤研究(C) 研究成果報告書 en : 1997 Fiscal Year Final Research Report 巻 1996-1997, p. 12p., 発行日 1998-03 |
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出版者 | ||||||
出版者 | 金沢大学医薬保健研究域医学系 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | 活性酸素のアポトーシスにおける重要性を活性酸素の産生欠如が主病因である慢性肉芽腫症(CGD)患児由来細胞を用い検討し,Fas誘導性アポトーシスにおける活性酸素の意義を検討した。正常ヒト由来細胞は抗Fas抗体の添加によりアポトーシス誘導を受けるのに対し、CGD由来細胞は抗Fas抗体の点かによりアポトーシス誘導を受けるのに対し、CGD由来細胞では僅かであった。カタラーゼを添加することにより産生されるH_2O_2を分解すると濃度依存性に抗Fas抗体のアポトーシス増強作用は抑制され,一方、CGD由来細胞をH_2O_2存在下で培養するとFas誘導性アポトーシスの増強が認められた.FasあるいはFasリガンドの遺伝子変異による自己免疫疾患モデル1prgldマウスと類似した肝脾腫リンパ節腫大、汎血球減少,溶血性貧血,高γグロブリン血症等の臨床像を呈するヒト自己免疫性リンパ球増殖症候群の兄妹例においてFas誘導製アポトーシスを解析したところ,患児由来細胞においては全くFas誘導性アポトーシスが認められなかった.Western blot解析にて異常Fas抗原分子が同定され,遺伝子解析の結果,Fas intron7におけるT→C置換により患児由来のFas抗原はdeathdomainを含む細胞内部分を欠如すること,さらにこの遺伝子異常は母親由来であることが判明した.患児及び母親の末消血においてTCRαβ+CD4-CD8-T細胞の増加が認められた.Fas抗原を介したアポトーシスの生体内での免疫学的恒常性における重要性が示唆された. | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | 1) The involvement of reactive oxygen intermediates in Fas-mediated apoptosis was investigated using cells from patients with chronic granulomatous disease (CGD) that have no ability of reactive oxygen intermediates production. Although the treatments with anti-Fas antibody induced apoptosis of cells from normal subjects, cells from CGD were resistant to anti-Fas antibody. Catalase significantly inhibited Fas-mediated apoptosis of normal cells, on the other hands hydrogen peroxides induced apoptosis of CGD cells. 2) Fas-mediated apoptosis was estimated in two siblings with autoimmune lymphoproliferative syndrome characterized with hepatosplenomegaly, lymphadenopathy, pancytopenia, hyper-gammagloblinemia, which is similar to clinicalmanifestations of lpr or gld mouse that have mutations of Fas or Fas ligand gene. Cells from two patients showed reduced Fas-induced apoptosis and smaller abnormal Fas protein was detected by western blot analysis. The T to C point mutation of intron 7 in patients Fas gene was detected and Fas antigen of patients lacks intracellular portion including death domain by this mutation TCRalphabeta+CD4-CD6- T cells were increased in blood I patients and analysis of family showed that mother has a same Fas mutation. These results indicated significance of Fas-mediated apoptosis in immunological homeostasis n vivo. |
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内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 研究課題/領域番号:08670862, 研究期間(年度):1996-1997 | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 出典:「Fas誘導性アポトーシス細胞死における活性酸素の意義」研究成果報告書 課題番号08670862 (KAKEN:科学研究費助成事業データベース(国立情報学研究所)) 本文データは著者版報告書より作成 |
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著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://kaken.nii.ac.jp/search/?qm=30204366 | |||||
関連名称 | https://kaken.nii.ac.jp/search/?qm=30204366 | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08670862/ | |||||
関連名称 | https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08670862/ | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-08670862/086708621997kenkyu_seika_hokoku_gaiyo/ | |||||
関連名称 | https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-08670862/086708621997kenkyu_seika_hokoku_gaiyo/ |