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温度可変高速AFMの開発と温度依存的ATPaseの構造機能相関の解明
https://doi.org/10.24517/00056741
https://doi.org/10.24517/0005674159db02bb-92e8-4a02-82d4-e692f88a3555
名前 / ファイル | ライセンス | アクション |
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SC-PR-UCHIHASHI-T-kaken 2018-6p.pdf (1.3 MB)
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Item type | 報告書 / Research Paper(1) | |||||
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公開日 | 2020-02-03 | |||||
タイトル | ||||||
タイトル | 温度可変高速AFMの開発と温度依存的ATPaseの構造機能相関の解明 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Development of variable-temperature high-speed AFM and its application to temperature-dependent ATPases | |||||
言語 | ||||||
言語 | jpn | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_18ws | |||||
資源タイプ | research report | |||||
ID登録 | ||||||
ID登録 | 10.24517/00056741 | |||||
ID登録タイプ | JaLC | |||||
著者 |
内橋, 貴之
× 内橋, 貴之 |
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著者別表示 |
Uchihashi, Takayuki
× Uchihashi, Takayuki |
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提供者所属 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 金沢大学理工研究域数物科学系 | |||||
書誌情報 |
平成29(2017)年度 科学研究費補助金 基盤研究(B) 研究成果報告書 en : 2017 Fiscal Year Final Research Report 巻 2015-04-01 - 2018-03-31, p. 6p., 発行日 2018-09-04 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | 室温から約45℃の温度制御可能な高速AFMシステムを開発した。実際に、41度で固相から液相に相転移する脂質二重膜(DPPC)を用いて高温観察の評価を行い、相転移温度以上で膜の流動性が増加し脂質二重膜の形状が変化する様子を確認できた。温度制御システムを使って、べん毛の輸送タンパク質の一部であるFliIの観察を行ったところ、モノマーから六量体形成していく過程やFliI六量体の構造が変化する様子も観察できた。シアノバクテリアの概日周期タンパク質であるKaiCとKaiAの結合解離過程の温度依存性の測定にも成功した。 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | We have developed variable-temperature high-speed atomic force microscopy (VT-HS-AFM) with which the experimental temperature can be controlled ranging from a room temperature to around 40 ℃. The performance of VT-HS-AFM was confirmed by observation of fluidity of DPPC lipid bilayer with the phase-transition temperature of 41℃ from gel phase to liquid crystal phase. We applied the VT-HS-AFM system to observation of a flagellar protein, FliI, which is an ATPase with the activity optimum temperature over 40℃. HS-AFM images captured oligomerization processes of the FliI monomers to the hexamer and also conformational changes of the oligomers. Also we observed temperature dependent interaction between KaiC and KaiA which are proteins responsible to the circadian rhythm of Cyanobacteria. | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 研究課題/領域番号:15H03540, 研究期間(年度):2015-04-01 - 2018-03-31 | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 出典:研究課題「温度可変高速AFMの開発と温度依存的ATPaseの構造機能相関の解明」課題番号15H03540 (KAKEN:科学研究費助成事業データベース(国立情報学研究所)) (https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-15H03540/15H03540seika/)を加工して作成 |
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著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://kaken.nii.ac.jp/search/?qm=30326300 | |||||
関連名称 | https://kaken.nii.ac.jp/search/?qm=30326300 | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15H03540/ | |||||
関連名称 | https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15H03540/ | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-15H03540/15H03540seika/ | |||||
関連名称 | https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-15H03540/15H03540seika/ |