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植物表皮プロテオミクスを用いた病原菌に対する侵入抵抗性の制御因子の探索と機能解析
https://doi.org/10.24517/00057600
https://doi.org/10.24517/00057600d2f01803-271e-4e64-b491-52eb1668c462
名前 / ファイル | ライセンス | アクション |
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AC-PR-NISHIUCHI-T-kaken 2021-9p.pdf (190.3 kB)
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Item type | 報告書 / Research Paper(1) | |||||
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公開日 | 2022-02-10 | |||||
タイトル | ||||||
タイトル | 植物表皮プロテオミクスを用いた病原菌に対する侵入抵抗性の制御因子の探索と機能解析 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Proteomic study of plant epidermal tissues in response to phytopathogen invasion | |||||
言語 | ||||||
言語 | jpn | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_18ws | |||||
資源タイプ | research report | |||||
ID登録 | ||||||
ID登録 | 10.24517/00057600 | |||||
ID登録タイプ | JaLC | |||||
著者別表示 |
Nishiuchi, Takumi
× Nishiuchi, Takumi |
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提供者所属 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 金沢大学学際科学実験センター遺伝子研究施設 | |||||
書誌情報 |
令和2(2020)年度 科学研究費補助金 基盤研究(C) 研究成果報告書 en : 2020 Fiscal Year Final Research Report 巻 2018-04-01 - 2021-03-31, p. 9p., 発行日 2021-06-11 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | 植物表層における病原菌との相互作用に関わるタンパク質を同定するため、シロイヌナズナの葉に赤かび病菌の胞子懸濁液を滴下接種した表皮組織を用いて、ショットガンプロテオーム解析を行った。接種4時間後の表皮において、MAPKKK等のシグナル伝達因子やタンパク質分解に関わるタンパク質の発現量が増加していた。接種24時間後の表皮では、転写因子、PRタンパク質の発現も増加していたが、葉全体ではこれらの発現増加は見られなかった。また、赤かび病菌のエフェクター様タンパク質も多く検出されたことから、接種表皮を用いたプロテオーム解析は、植物と病原菌の相互作用に関わるタンパク質の検出に有効であることが示された。 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | The plant epidermis is the first line of plant defence against pathogen invasion and likely contains important regulatory proteins related to the plant-pathogen interaction. We performed comparative proteomic studies to identify differentially expressed proteins in the leaf epidermis challenged with fungal phytopathogen. The conidia solutions were dropped onto the Arabidopsis leaf surface and then collect the epidermal tissues from inoculated and mock treated leaves at 4 and 24 hpi. Label free quantification methods showed that proteins related to defence signaling, transcription factors, and cell wall organization were up-regulated in the inoculated leaf epidermis. More than 300 fungal proteins expressed on the Arabidopsis epidermis were also identified and about 13% of them were predicted as effector-like proteins. These results suggested that our method is useful for understanding the early expressions of plant and fungal proteins related to their interactions. | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 研究課題/領域番号:18K05642, 研究期間(年度):2018-04-01 - 2021-03-31 | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 出典:「植物表皮プロテオミクスを用いた病原菌に対する侵入抵抗性の制御因子の探索と機能解析」研究成果報告書 課題番号18K05642 (KAKEN:科学研究費助成事業データベース(国立情報学研究所)) (https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-18K05642/18K05642seika/)を加工して作成 |
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著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://kaken.nii.ac.jp/search/?qm=20334790 | |||||
関連名称 | https://kaken.nii.ac.jp/search/?qm=20334790 | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18K05642/ | |||||
関連名称 | https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18K05642/ | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-18K05642/18K05642seika/ | |||||
関連名称 | https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-18K05642/18K05642seika/ |