WEKO3
インデックスリンク
アイテム
{"_buckets": {"deposit": "3e4d3edb-096a-4ec5-b4fc-1af0b435ab47"}, "_deposit": {"created_by": 18, "id": "51617", "owners": [18], "pid": {"revision_id": 0, "type": "depid", "value": "51617"}, "status": "published"}, "_oai": {"id": "oai:kanazawa-u.repo.nii.ac.jp:00051617", "sets": ["4014"]}, "author_link": ["77348", "210"], "item_9_biblio_info_8": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2021-05-17", "bibliographicIssueDateType": "Issued"}, "bibliographicPageStart": "16p.", "bibliographicVolumeNumber": "2017-04-01 - 2021-03-31", "bibliographic_titles": [{"bibliographic_title": "令和2(2020)年度 科学研究費補助金 基盤研究(B) 研究成果報告書"}, {"bibliographic_title": "2020 Fiscal Year Final Research Report", "bibliographic_titleLang": "en"}]}]}, "item_9_creator_33": {"attribute_name": "著者別表示", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Yamada, Masahito"}], "nameIdentifiers": [{"nameIdentifier": "77348", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "80191336", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=80191336"}]}]}, "item_9_description_21": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "チオフラビンT(ThT)分析及び高速原子間力顕微鏡観察によりオートクレーブ(AC)はアミロイドβ蛋白(Aβ)凝集体のseeding効果を低下させることを明らかにした。高温、長時間またはAβ凝集体濃度が低い条件でACした場合にAβ凝集体のseeding効果は低下しやすく、同じAC条件ではAβ40凝集体のseeding効果はAβ42凝集体のそれよりも低下しやすかった。ThT分析、円偏光二色性スペクトル測定及び電子顕微鏡の結果より、ACはAβ凝集体に含まれるβシート構造を減少させ、線維長を短縮させることを解明した。一方、γ線照射はAβ凝集体のseeding効果や構造には影響を与えなかった。", "subitem_description_type": "Abstract"}, {"subitem_description": "Studies with thioflavin T (ThT) assays and high-speed atomic force microscopy revealed that seeding effect of amyloid β-protein (Aβ) aggregates was inactivated by autoclave (AC), depending on different temperatures, exposure times, and concentrations of Aβ aggregates at the treatment with AC. Seeding activity of Aβ40 aggregates was more easily inactivated by AC at the same conditions compared to that of Aβ42 aggregates. Results of ThT fluorescence and circular dichroism spectral patterns of autoclaved Aβ aggregates indicated that AC reduced β-sheet-rich structures in Aβ aggregates. Electron microscopy images demonstrated that lengths of autoclaved Aβ aggregates with fibril-like structures was significantly shorter than those of non-treated ones. Gamma-ray irradiation for Aβ aggregates did not inactivate Aβ seeding effect or induce structural changes of Aβ aggregates significantly.", "subitem_description_type": "Abstract"}]}, "item_9_description_22": {"attribute_name": "内容記述", "attribute_value_mlt": [{"subitem_description": "研究課題/領域番号:17H04194, 研究期間(年度):2017-04-01 - 2021-03-31", "subitem_description_type": "Other"}, {"subitem_description": "出典:「Aβ凝集体の個体間伝播とその防御に関する研究」研究成果報告書 課題番号17H04194\n(KAKEN:科学研究費助成事業データベース(国立情報学研究所)) \n(https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-17H04194/17H04194seika/)を加工して作成", "subitem_description_type": "Other"}]}, "item_9_description_5": {"attribute_name": "提供者所属", "attribute_value_mlt": [{"subitem_description": "金沢大学医薬保健研究域医学系", "subitem_description_type": "Other"}]}, "item_9_identifier_registration": {"attribute_name": "ID登録", "attribute_value_mlt": [{"subitem_identifier_reg_text": "10.24517/00057920", "subitem_identifier_reg_type": "JaLC"}]}, "item_9_relation_28": {"attribute_name": "関連URI", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "https://kaken.nii.ac.jp/search/?qm=80191336"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://kaken.nii.ac.jp/search/?qm=80191336", "subitem_relation_type_select": "URI"}}, {"subitem_relation_name": [{"subitem_relation_name_text": "https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17H04194/"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17H04194/", "subitem_relation_type_select": "URI"}}, {"subitem_relation_name": [{"subitem_relation_name_text": "https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-17H04194/17H04194seika/"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-17H04194/17H04194seika/", "subitem_relation_type_select": "URI"}}]}, "item_9_version_type_25": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_ab4af688f83e57aa", "subitem_version_type": "AM"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "山田, 正仁"}], "nameIdentifiers": [{"nameIdentifier": "210", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "80191336", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=80191336"}, {"nameIdentifier": "80191336", "nameIdentifierScheme": "金沢大学研究者情報", "nameIdentifierURI": "http://ridb.kanazawa-u.ac.jp/public/detail.php?kaken=80191336"}, {"nameIdentifier": "80191336", "nameIdentifierScheme": "研究者番号", "nameIdentifierURI": "https://nrid.nii.ac.jp/nrid/1000080191336"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2022-02-17"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "ME-PR-YAMADA-M-kaken 2021-16p.pdf", "filesize": [{"value": "108.9 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_11", "mimetype": "application/pdf", "size": 108900.0, "url": {"label": "ME-PR-YAMADA-M-kaken 2021-16p.pdf", "url": "https://kanazawa-u.repo.nii.ac.jp/record/51617/files/ME-PR-YAMADA-M-kaken 2021-16p.pdf"}, "version_id": "f6f2f156-3f57-4002-940f-256305a56404"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "アルツハイマー病", "subitem_subject_scheme": "Other"}, {"subitem_subject": "アミロイドβ蛋白", "subitem_subject_scheme": "Other"}, {"subitem_subject": "伝播", "subitem_subject_scheme": "Other"}, {"subitem_subject": "防御", "subitem_subject_scheme": "Other"}, {"subitem_subject": "凝集", "subitem_subject_scheme": "Other"}, {"subitem_subject": "オートクレーブ処理", "subitem_subject_scheme": "Other"}, {"subitem_subject": "γ線照射", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "jpn"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "research report", "resourceuri": "http://purl.org/coar/resource_type/c_18ws"}]}, "item_title": "Aβ凝集体の個体間伝播とその防御に関する研究", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Aβ凝集体の個体間伝播とその防御に関する研究"}, {"subitem_title": "Study on transmission of Abeta aggregates and its prevention", "subitem_title_language": "en"}]}, "item_type_id": "9", "owner": "18", "path": ["4014"], "permalink_uri": "https://doi.org/10.24517/00057920", "pubdate": {"attribute_name": "公開日", "attribute_value": "2022-02-17"}, "publish_date": "2022-02-17", "publish_status": "0", "recid": "51617", "relation": {}, "relation_version_is_last": true, "title": ["Aβ凝集体の個体間伝播とその防御に関する研究"], "weko_shared_id": -1}
Aβ凝集体の個体間伝播とその防御に関する研究
https://doi.org/10.24517/00057920
https://doi.org/10.24517/00057920a97f5ed0-44b8-4a8f-a0e9-0b54c4889c6e
名前 / ファイル | ライセンス | アクション |
---|---|---|
ME-PR-YAMADA-M-kaken 2021-16p.pdf (108.9 kB)
|
Item type | 報告書 / Research Paper(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2022-02-17 | |||||
タイトル | ||||||
タイトル | Aβ凝集体の個体間伝播とその防御に関する研究 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Study on transmission of Abeta aggregates and its prevention | |||||
言語 | ||||||
言語 | jpn | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_18ws | |||||
資源タイプ | research report | |||||
ID登録 | ||||||
ID登録 | 10.24517/00057920 | |||||
ID登録タイプ | JaLC | |||||
著者 |
山田, 正仁
× 山田, 正仁 |
|||||
著者別表示 |
Yamada, Masahito
× Yamada, Masahito |
|||||
提供者所属 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 金沢大学医薬保健研究域医学系 | |||||
書誌情報 |
令和2(2020)年度 科学研究費補助金 基盤研究(B) 研究成果報告書 en : 2020 Fiscal Year Final Research Report 巻 2017-04-01 - 2021-03-31, p. 16p., 発行日 2021-05-17 |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | チオフラビンT(ThT)分析及び高速原子間力顕微鏡観察によりオートクレーブ(AC)はアミロイドβ蛋白(Aβ)凝集体のseeding効果を低下させることを明らかにした。高温、長時間またはAβ凝集体濃度が低い条件でACした場合にAβ凝集体のseeding効果は低下しやすく、同じAC条件ではAβ40凝集体のseeding効果はAβ42凝集体のそれよりも低下しやすかった。ThT分析、円偏光二色性スペクトル測定及び電子顕微鏡の結果より、ACはAβ凝集体に含まれるβシート構造を減少させ、線維長を短縮させることを解明した。一方、γ線照射はAβ凝集体のseeding効果や構造には影響を与えなかった。 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Studies with thioflavin T (ThT) assays and high-speed atomic force microscopy revealed that seeding effect of amyloid β-protein (Aβ) aggregates was inactivated by autoclave (AC), depending on different temperatures, exposure times, and concentrations of Aβ aggregates at the treatment with AC. Seeding activity of Aβ40 aggregates was more easily inactivated by AC at the same conditions compared to that of Aβ42 aggregates. Results of ThT fluorescence and circular dichroism spectral patterns of autoclaved Aβ aggregates indicated that AC reduced β-sheet-rich structures in Aβ aggregates. Electron microscopy images demonstrated that lengths of autoclaved Aβ aggregates with fibril-like structures was significantly shorter than those of non-treated ones. Gamma-ray irradiation for Aβ aggregates did not inactivate Aβ seeding effect or induce structural changes of Aβ aggregates significantly. | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 研究課題/領域番号:17H04194, 研究期間(年度):2017-04-01 - 2021-03-31 | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 出典:「Aβ凝集体の個体間伝播とその防御に関する研究」研究成果報告書 課題番号17H04194 (KAKEN:科学研究費助成事業データベース(国立情報学研究所)) (https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-17H04194/17H04194seika/)を加工して作成 |
|||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://kaken.nii.ac.jp/search/?qm=80191336 | |||||
関連名称 | https://kaken.nii.ac.jp/search/?qm=80191336 | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17H04194/ | |||||
関連名称 | https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17H04194/ | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-17H04194/17H04194seika/ | |||||
関連名称 | https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-17H04194/17H04194seika/ |