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人工変異体を用いたヘモグロビンの引き金説の検証および酸素運搬分子メカニズムの解明
https://doi.org/10.24517/00059923
https://doi.org/10.24517/0005992305444a30-9093-495f-b67d-0ec97a14d356
名前 / ファイル | ライセンス | アクション |
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ME-PR-AKI-Y-kaken 2011-5p.pdf (345.2 kB)
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Item type | 報告書 / Research Paper(1) | |||||
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公開日 | 2020-11-30 | |||||
タイトル | ||||||
タイトル | 人工変異体を用いたヘモグロビンの引き金説の検証および酸素運搬分子メカニズムの解明 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Oxygen carrying mechanism of hemoglobin : verification of trigger model by using proximal cavity mutant hemoglobin | |||||
言語 | ||||||
言語 | jpn | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_18ws | |||||
資源タイプ | research report | |||||
ID登録 | ||||||
ID登録 | 10.24517/00059923 | |||||
ID登録タイプ | JaLC | |||||
著者 |
安藝, 弥生
× 安藝, 弥生 |
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著者別表示 |
Aki, Yayoi
× Aki, Yayoi |
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提供者所属 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 金沢大学医薬保健研究域医学系 | |||||
書誌情報 |
平成22(2010)年度 科学研究費補助金 基盤研究(C) 研究成果報告書 en : 2010 Fiscal Year Final Research Report 巻 2007 – 2010, p. 5p., 発行日 2011-05-17 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | ヘモグロビンの四次構造変化は、ヘム鉄と唯一結合している近位Hisの変化がきっかけで引き起こされると信じられている(引き金説)。本研究は、α鎖またはβ鎖グロビンの近位HisをGlyに置換した人工変異ヘモグロビンを合成し、それらの構造変化・酸素運搬機能について解析し、引き金説を直接検証した。その結果、α鎖の近位Hisの変化はヘモグロビンの四次構造変化の引き金になることが示されたが、β鎖の方はなくても四次構造変化は不完全ながらおこるので、引き金ではないことが示された。 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Many people believe that the trigger of the quaternary structural transition of hemoglobin (Hb) is the displacement of the proximal His, which is the only amino acid binding directly to the heme iron (trigger model). I inspected the model by synthesizing the recombinant Hbs, in which the proximal His is replaced with Gly in the α or β subunit, and then analyzing the conformational change and the oxygen carrying mechanism of these Hbs. I have suggested that the shift of the proximal His in the α subunit is the trigger of the structural transition of Hb, whereas one of the β subunit is not the trigger because the conformational change is partially observed in the recombinant Hb without the β proximal His. | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 研究課題/領域番号:19579002, 研究期間(年度):2007 – 2010 | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 出典:研究課題「人工変異体を用いたヘモグロビンの引き金説の検証および酸素運搬分子メカニズムの解明」課題番号19579002 (KAKEN:科学研究費助成事業データベース(国立情報学研究所)) (https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-19579002/19579002seika/)を加工して作成 |
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著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://kaken.nii.ac.jp/ja/search/?kw=00452110 | |||||
関連名称 | https://kaken.nii.ac.jp/ja/search/?kw=00452110 | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-19579002/ | |||||
関連名称 | https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-19579002/ | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-19579002/19579002seika/ | |||||
関連名称 | https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-19579002/19579002seika/ |