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Isolation of Testosterone-binding Globulin from Bovine Serum by Affinity Chromatography and Its Molecular Characterization
https://doi.org/10.24517/00060080
https://doi.org/10.24517/00060080bcc80c77-7ac5-4b4f-9fac-7711fe7ca78c
名前 / ファイル | ライセンス | アクション |
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SC-PR-ITAGAKI-E-81_1721.pdf (1.4 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2020-12-03 | |||||
タイトル | ||||||
タイトル | Isolation of Testosterone-binding Globulin from Bovine Serum by Affinity Chromatography and Its Molecular Characterization | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
ID登録 | ||||||
ID登録 | 10.24517/00060080 | |||||
ID登録タイプ | JaLC | |||||
著者 |
Suzuki, Yasuyuki
× Suzuki, Yasuyuki× Itagaki, Eiji× Mori, Hiromu× Hosoya, Toichiro |
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著者別表示 |
板垣, 英治
× 板垣, 英治 |
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書誌情報 |
The Journal of Biochemistry 巻 81, 号 6, p. 1721-1731, 発行日 1977 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0021-924X | |||||
NCID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA00694073 | |||||
出版者 | ||||||
出版者 | The Japanese Biochemical Society | |||||
出版者(別名) | ||||||
出版者 | 日本生化学会 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | The testosterone-binding globulin (TeBG) from bovine serum was purified by affinity chromatography and hydroxylapatite chromatography. The affinity column used was prepared by coupling 17α-carboxyethynyl-l7-hydroxy-4-androsten-3-one to aminoethyl-Sepharose. The compound was replaceable by 17α-carboxyethynyl-l7-hydroxy-5α-androstan-3-one, but not by testosterone 17-hemisuccinate, estradiol 17-hemisuccinate, or testosterone 3-(O-carboxy-methyl)oxime. The TeBG isolated was homogeneous on analytical polyacrylamide gel electrophoresis and equilibrium centrifugation. The protein was a glycoprotein having a molecular weight of 89, 500 and a carbohydrate content of 17%. The association constant (M-1) at 4°C was 1.1 × 108 and the number of binding sites per molecule was 0.8. Treatment with guanidine-HCI dissociated the protein into subunits having a molecular weight of 28, 400 (about one-third of that of the original molecule). SDS-gel electrophoresis showed that two of the three subunits were slightly larger than the other. The dissociation into subunits could also be accomplished by GEDTA treatment with concomitant loss of testosterone-binding activity. The activity and molecular size were reversibly restored by incubation with excess Ca2+. | |||||
権利 | ||||||
権利情報 | Copyright © The Japanese Biochemical Society 日本生化学会 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | http://www.jbsoc.or.jp/ | |||||
関連名称 | http://www.jbsoc.or.jp/ | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://academic.oup.com/jb | |||||
関連名称 | https://academic.oup.com/jb | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://www.jstage.jst.go.jp/browse/biochemistry1922/-char/ja | |||||
関連名称 | https://www.jstage.jst.go.jp/browse/biochemistry1922/-char/ja |