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Characterization of CD133+ parenchymal cells in the liver: Histology and culture
https://doi.org/10.24517/00061735
https://doi.org/10.24517/00061735b61cde91-b6f3-4a22-9adf-bf8ef2384bbf
名前 / ファイル | ライセンス | アクション |
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ME-PR-NAKANUMA-Y-15-4896.pdf (1.5 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2021-08-30 | |||||
タイトル | ||||||
タイトル | Characterization of CD133+ parenchymal cells in the liver: Histology and culture | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
ID登録 | ||||||
ID登録 | 10.24517/00061735 | |||||
ID登録タイプ | JaLC | |||||
著者 |
Yoshikawa, Seiichi
× Yoshikawa, Seiichi× Zen, Yoh× Fujii, Takahiko× Sato, Yasunori× Ohta, Tetsuo× Aoyagi, Yutaka× Nakanuma, Yasuni |
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著者別表示 |
全, 陽
× 全, 陽× 佐藤, 保則× 太田, 哲生× 中沼, 安二 |
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提供者所属 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 金沢大学医薬保健研究域医学系 | |||||
書誌情報 |
World Journal of Gastroenterology 巻 15, 号 39, p. 4896-4906, 発行日 2009 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1007-9327 | |||||
NCID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA12048247 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.3748/wjg.15.4896 | |||||
出版者 | ||||||
出版者 | Baishideng Publishing Group Co | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | AIM: To reveal the characteristics of CD133+ cells in the liver. METHODS: This study examined the histological characteristics of CD133 + cells in non-neoplastic and neoplastic liver tissues by immunostaining, and also analyzed the biological characteristics of CD133 + cells derived from human hepatocellular carcinoma (HCC) or cholangiocarcinoma cell lines. RESULTS: Immunostaining revealed constant expression of CD133 in non-neoplastic and neoplastic biliary epithelium, and these cells had the immunophenotype CD133+/CK19+/HepPar -1-. A smal l number of CD133+/CK19-/HepPar-1+ cells were also identified in HCC and combined hepatocellular and cholangiocarcinoma. In addition, small ductal structures, resembling the canal of Hering, partly surrounded by hepatocytes were positive for CD133. CD133 expression was observed in three HCC (HuH7, PLC5 and HepG2) and two cholangiocarcinoma cell lines (HuCCT1 and CCKS1). Fluorescence-activated cell sorting (FACS) revealed that CD133+ and CD133-cells derived from HuH7 and HuCCT1 cells similarly produced CD133+ and CD133- cells during subculture. To examine the relationship between CD133+ cells and the side population (SP) phenotype, FACS was performed using Hoechst 33342 and a monoclonal antibody against CD133. The ratios of CD133+/CD133-cells were almost identical in the SP and non-SP in HuH7. In addition, four different cellular populations (SP/CD133+, SP/CD133-, non-SP/CD133+, and non-SP/CD133-) could similarly produce CD133+ and CD133- cells during subculture. CONCLUSION: This study revealed that CD133 could be a biliary and progenitor cell marker in vivo. However, CD133 alone is not sufficient to detect tumor-initiating cells in cell lines. © 2009 The WJG Press and Baishideng. All rights reserved. | |||||
権利 | ||||||
権利情報 | Copyright ©The Author(s). 2009 Published by Baishideng Publishing Group Inc. All rights reserved. | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://www.wjgnet.com/ | |||||
関連名称 | https://www.wjgnet.com/ | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://www.wjgnet.com/1007-9327/index.htm | |||||
関連名称 | https://www.wjgnet.com/1007-9327/index.htm |