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Tumor Endothelial Cell-Specific Drug Delivery System Using Apelin-Conjugated Liposomes
https://doi.org/10.24517/00062986
https://doi.org/10.24517/0006298678c97498-404c-4bdb-88d5-ae0f0cd91042
名前 / ファイル | ライセンス | アクション |
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ME-PR-NAITO-H-e65499.pdf (265.5 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2021-07-05 | |||||
タイトル | ||||||
タイトル | Tumor Endothelial Cell-Specific Drug Delivery System Using Apelin-Conjugated Liposomes | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
ID登録 | ||||||
ID登録 | 10.24517/00062986 | |||||
ID登録タイプ | JaLC | |||||
著者 |
Kawahara, Hiroki
× Kawahara, Hiroki× Naito, Hisamichi× Takara, Kazuhiro× Wakabayashi, Taku× Kidoya, Hiroyasu× Takakura, Nobuyuki |
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著者別表示 |
内藤, 尚道
× 内藤, 尚道× 高倉, 伸幸 |
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提供者所属 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 金沢大学医薬保健研究域医学系 | |||||
書誌情報 |
PLoS ONE 巻 8, 号 6, p. e65499, 発行日 2013-06-14 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1932-6203 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1371/journal.pone.0065499 | |||||
出版者 | ||||||
出版者 | Public Library of Science | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Background:A drug delivery system specifically targeting endothelial cells (ECs) in tumors is required to prevent normal blood vessels from being damaged by angiogenesis inhibitors. The purpose of this study was to investigate whether apelin, a ligand for APJ expressed in ECs when angiogenesis is taking place, can be used for targeting drug delivery to ECs in tumors.Methods and Results:Uptake of apelin via APJ stably expressed in NIH-3T3 cells was investigated using TAMRA (fluorescent probe)-conjugated apelin. Both long and short forms of apelin (apelin 36 and apelin 13) were taken up, the latter more effectively. To improve efficacy of apelin- liposome conjugates, we introduced cysteine, with its sulfhydryl group, to the C terminus of apelin 13, resulting in the generation of apelin 14. In turn, apelin 14 was conjugated to rhodamine-encapsulating liposomes and administered to tumor-bearing mice. In the tumor microenvironment, we confirmed that liposomes were incorporated into the cytoplasm of ECs. In contrast, apelin non-conjugated liposomes were rarely found in the cytoplasm of ECs. Moreover, non-specific uptake of apelin-conjugated liposomes was rarely detected in other normal organs.Conclusions:ECs in normal organs express little APJ; however, upon hypoxic stimulation, such as in tumors, ECs start to express APJ. The present study suggests that apelin could represent a suitable tool to effectively deliver drugs specifically to ECs within tumors. © 2013 Kawahara et al. | |||||
権利 | ||||||
権利情報 | copyright © Public Library of Science | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | http://www.plosone.org/ | |||||
関連名称 | http://www.plosone.org/ | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0065499 | |||||
関連名称 | https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0065499 |