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培養細胞へのCryptosporidium parvumの感染とELISAによる検出
https://doi.org/10.24517/00064539
https://doi.org/10.24517/00064539c8479014-bb29-4f97-a965-69d8c6bacbd2
名前 / ファイル | ライセンス | アクション |
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TE-PR-MIKI-O-23_427.pdf (805.3 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2022-01-24 | |||||
タイトル | ||||||
タイトル | 培養細胞へのCryptosporidium parvumの感染とELISAによる検出 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Infection of Cryptosporidium parvum Oocysts to Cultured Cells and Detection of the Oocysts by ELISA | |||||
言語 | ||||||
言語 | jpn | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
ID登録 | ||||||
ID登録 | 10.24517/00064539 | |||||
ID登録タイプ | JaLC | |||||
著者 |
加藤, 敏朗
× 加藤, 敏朗× 竹内, りさ× 小野, 芳朗× 三木, 理× 河原, 長美 |
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著者別表示 |
Kato, Toshiaki
× Kato, Toshiaki× Takeuchi, Lisa× Ono, Yoshihiro× 三木, 理× Kawara, Osami |
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提供者所属 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 金沢大学理工研究域機械工学系 | |||||
書誌情報 |
水環境学会誌 en : Journal of Japan Society on Water Environment 巻 23, 号 7, p. 427-432, 発行日 2000 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0916-8958 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1881-3690 | |||||
NCID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN10372439 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.2965/jswe.23.427 | |||||
出版者 | ||||||
出版者 | 日本水環境学会 Japan Society on Water Environment | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | For developing water treatment system to prevent waterborne Cryptosporidiosis, it is necessary to estimate the inactivation of Cryptosporidium. Recently, cell culture model has developed into a tool that can be used for assessing the viability and/or infectivity of Cryptosporidium oocysts. In this study, we investigated to develop quantative and sensitive detection method for determining the infectivity of Cryptosporidium oocysts by combining cell culture with enzyme-linked immunosorbent assay (ELISA). Human ileocecal epitherial cells (HCT-8), which were seeded in gelatin-coated 96-well plates at the density of 1 × 104 cells · well-1 and incubated at 37°C for 48h, were infected with the oocysts. After inoculation, HCT-8 cells were re-incubated with fresh culture media at 37°C for 48h, then were processed for ELISA. The absorbance values in ELISA were increased in an inoculating dose-dependent manner. In the experimental condition, the number of the oocysts from 1 × 102 to 3 × 104 per well was quantitatively detected. | |||||
権利 | ||||||
権利情報 | Copyright © 日本水環境学会 Japan Society on Water Environment | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://www.jstage.jst.go.jp/browse/jswe/-char/ja/ | |||||
関連名称 | https://www.jstage.jst.go.jp/browse/jswe/-char/ja/ | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | http://www.jswe.or.jp/index-j.html | |||||
関連名称 | http://www.jswe.or.jp/index-j.html |