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異常血色素における機能異常とタンパク質の高次構造変化との関連
https://doi.org/10.24517/00066297
https://doi.org/10.24517/000662975b8a2ff9-ba17-4da6-8540-0e202b334e64
名前 / ファイル | ライセンス | アクション |
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ME-PR-NAGAI-M-kaken 1999-2p.pdf (87.3 kB)
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Item type | 報告書 / Research Paper(1) | |||||
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公開日 | 2022-06-03 | |||||
タイトル | ||||||
タイトル | 異常血色素における機能異常とタンパク質の高次構造変化との関連 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Relation between the higher order structural changes of protein and function abnormarity of abnormal hemoglobin | |||||
言語 | ||||||
言語 | jpn | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_18ws | |||||
資源タイプ | research report | |||||
ID登録 | ||||||
ID登録 | 10.24517/00066297 | |||||
ID登録タイプ | JaLC | |||||
著者 |
長井, 雅子
× 長井, 雅子 |
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著者別表示 |
Nagai, Masako
× Nagai, Masako |
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提供者所属 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 金沢大学医学部 | |||||
書誌情報 |
平成9(1997)年度 科学研究費補助金 基盤研究(C) 研究成果報告書概要 en : 1997 Fiscal Year Final Research Report Summary 巻 1995 – 1997, p. 2p., 発行日 1999-03-15 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | 235nm励起紫外共鳴ラマン(UVRR)では主にチロシン(Tyr)とトリプトファン(Trp)に関する情報が得られる。ヘモグロビン(Hb)がdeoxy形(T)からCO形(R)に変わるとき、Trpでは主に強度変化が、Tyrでは波数シフトと強度変化の両方が起こった(J.Biol.Chem.,1995)。UVRRのTyrやTrpの変化とdeoxyHbの負のCD帯の出現にはサブユニット接触面(α_1β_2)の芳香族アミノ酸の関与が考えられるので、この部位のTyrやTrpが他のアミノ酸に変異した異常Hbおよび遺伝子工学的手法による人工変異Hbを用いて測定しその影響を調べた(J.Mol.Struct.,1966)。UVRRでは蛋白中のTyr残基を浮き彫りにしてその構造変化を明らかに出来るので、シグナル伝達やがん遺伝子などに関係するチロシンキナーゼによるチロシンリン酸化をヘモグロビン研究と平行して研究対象とした(J.Raman Spectrosc.,1998)。また、ヘモグロビンのT→R転移における構造変化をNOHbを用いて検索した。UVRRにみられる変化がリガンド結合そのものに由来するのか、真に四次構造変化に起因するのかを知る目的である。NOHbはリガンド結合の有無に関係なく、アロステリックエフェクター(IHP)の添加でT構造に変換することが知られているからである。その結果、UVRRでみている変化は真に四次構造変化を反映しているという興味ある結果を得た(現在、論文投稿中)。 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | It has been recently reported that resonance Raman spectra excited in the UV region at 200-240 can explore the environmental and hydrogen bonding changes of tryptophan (Trp) and tyrosine (Tyr) residues of proteins. we have demonstrated here the quaternary-structural dependent features for Tyr and Trp residues in alpha_1beta_2 interface of hemoglobin (Hb) from 235-nm excited UV resonance Raman (UVRR) spectra. Deoxy Hb A (T-form) showd a UVRR spectrum distinctly different from those of the ligated Hbs (R-form) including osyHb, COHb and NOHb. To characterize the spectral changes of Trp-beta37 at alpha_1beta_2 interface due to the quaternary structure transition, the UVRR spectra of Hb A were compared with the corresponding spectra of Hb Hirose (Trp-beta37->Ser). Comparison of the Hb A - Hb Hirose difference spectra in oxy and deoxy states revealed that the oxygenation- induced changes of Trp RR bands arose mostly from Trp-beta37. The UVRR spectral contribution of alpha42Tyr, Which is located in the "swhitch" region of the alpha_1beta_2 interface and forms an H-bond with the carboxylate side chain of beta99 Asp only in the T-state, was deduced for each of the deoxy- and CO-forms by subtracting the spectra of Hb alpha Y42H from those of Hb A.This suggested that alpha42Tyr is responsible for the frequency shift of Y8a (1617cm^<-1>) and Y9a (1179cm^<-1>) of the Tyr RR bands of Hb alphaY42H upon quaternary structure change are alike. The extent of the oxidation of Hb M Sakatoon and Hb M Boston in the patients blood was determined by measurement of the intensity of EPR signal at g=6.0 for the normal subunits, g=6.7for the mutant subunit of Hb M Saskatoon, and g=6.3 for those of Hb M Boston. About 50% and 76% of mutant subunits in Hb M Boston and Hb M Sakatoon remained reduced in the fresh blood, respectively. |
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内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 研究課題/領域番号:07670140, 研究期間(年度):1995 – 1997 | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 出典:研究課題「異常血色素における機能異常とタンパク質の高次構造変化との関連」課題番号07670140 (KAKEN:科学研究費助成事業データベース(国立情報学研究所)) (https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-07670140/076701401997kenkyu_seika_hokoku_gaiyo/)を加工して作成 |
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著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://kaken.nii.ac.jp/search/?qm=60019578 | |||||
関連名称 | https://kaken.nii.ac.jp/search/?qm=60019578 | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07670140/ | |||||
関連名称 | https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07670140/ | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-07670140/076701401997kenkyu_seika_hokoku_gaiyo/ | |||||
関連名称 | https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-07670140/076701401997kenkyu_seika_hokoku_gaiyo/ |