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A replication-competent smallpox vaccine LC16m8Δ-based COVID-19 vaccine
https://doi.org/10.24517/00067276
https://doi.org/10.24517/00067276a2064c1e-0bb2-4b21-be45-a9906865aee6
名前 / ファイル | ライセンス | アクション |
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PH-PR-SAKAMOTO-A-11-2359.pdf (2.7 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2022-10-17 | |||||
タイトル | ||||||
タイトル | A replication-competent smallpox vaccine LC16m8Δ-based COVID-19 vaccine | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
ID登録 | ||||||
ID登録 | 10.24517/00067276 | |||||
ID登録タイプ | JaLC | |||||
著者 |
Sakamoto, Akihiko
× Sakamoto, Akihiko× Osawa, Hiroaki× Hashimoto, Hinata× Mizuno, Tetsushi× Hasyim, Ammar A.× Abe, Yu-ichi× Okahashi, Yuto× Ogawa, Ryohei× Iyori, Mitsuhiro× Shida, Hisatoshi× Yoshida, Shigeto |
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著者別表示 |
坂本, 明彦
× 坂本, 明彦× 水野, 哲志× 伊從, 光洋× 吉田, 栄人 |
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提供者所属 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 金沢大学医薬保健研究域薬学系 | |||||
書誌情報 |
Emerging Microbes & Infections 巻 11, 号 1, p. 2359-2370, 発行日 2022-09-29 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 2222-1751 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1080/22221751.2022.2122580 | |||||
出版者 | ||||||
出版者 | Taylor and Francis | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Viral vectors are a potent vaccine platform for inducing humoral and T-cell immune responses. Among the various viral vectors, replication-competent ones are less commonly used for coronavirus disease 2019 (COVID-19) vaccine development compared with replication-deficient ones. Here, we show the availability of a smallpox vaccine LC16m8Δ (m8Δ) as a replication-competent viral vector for a COVID-19 vaccine. M8Δ is a genetically stable variant of the licensed and highly effective Japanese smallpox vaccine LC16m8. Here, we generated two m8Δ recombinants: one harbouring a gene cassette encoding the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike (S) glycoprotein, named m8Δ-SARS2(P7.5-S)-HA; and one encoding the S protein with a highly polybasic motif at the S1/S2 cleavage site, named m8Δ-SARS2(P7.5-SHN)-HA. M8Δ-SARS2(P7.5-S)-HA induced S-specific antibodies in mice that persisted for at least six weeks after a homologous boost immunization. All eight analysed serum samples displayed neutralizing activity against an S-pseudotyped virus at a level similar to that of serum samples from patients with COVID-19, and more than half (5/8) also had neutralizing activity against the Delta/B.1.617.2 variant of concern. Importantly, most serum samples also neutralized the infectious SARS-CoV-2 Wuhan and Delta/B.1.617.2 strains. In contrast, immunization with m8Δ-SARS2(P7.5-SHN)-HA elicited significantly lower antibody titres, and the induced antibodies had less neutralizing activity. Regarding T-cell immunity, both m8Δ recombinants elicited S-specific multifunctional CD8+ and CD4+ T-cell responses even after just a primary immunization. Thus, m8Δ provides an alternative method for developing a novel COVID-19 vaccine. | |||||
権利 | ||||||
権利情報 | Copyright © 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group, on behalf of Shanghai Shangyixun Cultural Communication Co., Ltd | |||||
権利 | ||||||
権利情報 | This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://www.tandfonline.com/doi/full/10.1080/22221751.2022.2122580 | |||||
関連名称 | https://www.tandfonline.com/doi/full/10.1080/22221751.2022.2122580 | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://www.tandfonline.com/journals/TEMI | |||||
関連名称 | https://www.tandfonline.com/journals/TEMI | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | http://www.tandf.co.uk/journals/default.asp | |||||
関連名称 | http://www.tandf.co.uk/journals/default.asp |