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水溶液中におけるダブシルアミノ酸の会合挙動
http://hdl.handle.net/2297/14504
http://hdl.handle.net/2297/14504c0010785-52e2-41da-be7f-485197a928e2
名前 / ファイル | ライセンス | アクション |
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TE-PR-KUNIMOTO-K-799.pdf (419.7 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2017-10-03 | |||||
タイトル | ||||||
タイトル | 水溶液中におけるダブシルアミノ酸の会合挙動 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Aggregation behavior of dabsylated amino acids in aqueous solution | |||||
言語 | ||||||
言語 | jpn | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
林, 宏成
× 林, 宏成× 佐々木, 千鶴× 国本, 浩喜× 前田, 史郎× 細井, 信造× 桑江, 彰夫× 花井, 一彦 |
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提供者所属 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 金沢大学理工研究域物質化学系 | |||||
書誌情報 |
Bunseki Kagaku = 日本分析 巻 54, 号 9, p. 799-805, 発行日 2005-01-01 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0525-1931 | |||||
NCID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN00222633 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.2116/bunsekikagaku.54.799 | |||||
出版者 | ||||||
出版者 | 日本分析化学会 = Japan Society for Analytical Chemistry | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | ダブシルクロリド(Dabs-Cl)は,高速液体クロマトグラフィーなどによるタンパク質分析の際のアミノ酸標識剤である.著者らは,Dabs-Clで標識された一連のダブシル化アミノ酸(Dabs-AA)を合成し,紫外可視吸収スペクトル及び円二色性(CD)スペクトルを測定し,Dabs-AAの水溶液中での会合挙動を検討した.希薄水溶液では,Dabs-AAの可視部の吸収はジメチルアミノアゾベンゼン色素特有のpH依存性を示す.Dabs-AAの濃度上昇に伴い,500 nm付近のDabs-AAのモノマー由来の吸収は減少し,360~400 nm領域にダイマーに由来するピークが観測される.ダイマーの形成は共溶媒として用いたジメチルスルホキシド濃度,溶液温度及びアミノ酸側鎖(R)に依存する.Dabs-L-Pheの363 nmに観測されるダイマーの吸収帯に対応して,379 nmに負の第一コットン効果,359 nmに正の第二コットン効果をもつ強い誘起分裂型CDスペクトルが観測された.これらの結果から水溶液中では,Dabs-L-Pheは負のキラリティーを示す配向でダイマーを形成していることが示唆された. A series of dabsylated amino acids (Dabs-AA) were prepared and their aggregation behavior in aqueous solution was studied by UV/Vis absorption and CD spectroscopies. Dabs-AAs show pH- dependent absorption in the visible region, characteristic of the dimethylamio azobenzene chromophore in a dilute aqueous solution. Upon increasing the concentration of Dabs-AAs, the visible absorption around 500 nm of the Dabs-AA monomer decreased its intensity, and a new band due to the Dabs-AA dimer appeared in the 360-400 nm region. Dimer formation depended on the DMSO co-solvent concentration, the solution temperature and the R-groups of amino acids. A split-type induced CD spectrum was observed for the dimer absorption band of Dabs-L- Phe at 363 nm: a negative first Cotton effect at 379 nm and a positive second Cotton effect at 359 nm. These results indicate that a pair of Dabs-L-Phe molecules were stacked with negative exciton chirality. © 2005 The Japan Society for Analytical Chemistry. | |||||
権利 | ||||||
権利情報 | Copyright (c) 2005 by The Japan Society for Analytical Chemistry | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
関連URI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | http://dx.doi.org/10.2116/bunsekikagaku.54.799 | |||||
関連URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | http://www.jstage.jst.go.jp/article/bunsekikagaku/54/9/54_799/_article/-char/ja |