2026-03-12T14:22:51Z https://kanazawa-u.repo.nii.ac.jp/oai

oai:kanazawa-u.repo.nii.ac.jp:00010335 2024-06-20T06:02:49Z 934:935:937

Cytoplasmic tail-dependent internalization of membrane-type 1 matrix metalloproteinase is important for its invasion-promoting activity Uekita, Takamasa Itoh, Yoshifumi Yana, Ikuo 50233226 Ohno, Hiroshi 10154634 Seiki, Motoharu Membrane-type 1 matrix metalloproteinase (MT1-MMP) is an integral membrane proteinase that degrades the pericellular extracellular matrix (ECM) and is expressed in many migratory cells, including invasive cancer cells. MT1-MMP has been shown to localize at the migration edge and to promote cell migration; however, it is not clear how the enzyme is regulated during the migration process. Here, we report that MT1-MMP is internalized from the surface and that this event depends on the sequence of its cytoplasmic tail. Di-leucine (Leu571–572 and Leu578–579) and tyrosine573 residues are important for the internalization, and the µ2 subunit of adaptor protein 2, a component of clathrin-coated pits for membrane protein internalization, was found to bind to the LLY573 sequence. MT1-MMP was internalized predominantly at the adherent edge and was found to colocalize with clathrin-coated vesicles. The mutations that disturb internalization caused accumulation of the enzyme at the adherent edge, though the net proteolytic activity was not affected much. Interestingly, whereas expression of MT1-MMP enhances cell migration and invasion, the internalization-defective mutants failed to promote either activity. These data indicate that dynamic turnover of MT1-MMP at the migration edge by internalization is important for proper enzyme function during cell migration and invasion. 金沢大学自然科学研究科 理化学研究所・横浜研究所　免疫アレルギー科学総合研究センター(RCAI) 横浜市立大学大学院国際総合科学研究科生体超分子科学専攻　客員教授 Rockefeller University Press 2001-12-24 2017-10-03 eng journal article VoR http://hdl.handle.net/2297/11854 https://kanazawa-u.repo.nii.ac.jp/records/10335 10.1083/jcb.200108112 AA00694812 0021-9525 Journal of Cell Biology 155 7 1345 1356 https://kanazawa-u.repo.nii.ac.jp/record/10335/files/NS-PR-OHNO-M-1356.pdf application/pdf 500.7 kB 2017-10-03