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oai:kanazawa-u.repo.nii.ac.jp:00009631 2024-06-20T06:46:56Z 934:935:936

Direct HPILC Analysis on Cellulose Depolymerisation in Ionic Liquids 10748891 Kuroda, Kosuke Fukaya, Yukinobu Ohno, Hiroyuki Changes of average molecular weight and molecular weight distribution of cellulose in a polar ionic liquid (IL) were analysed with high performance liquid chromatography using a polar IL as an eluent (HPILC). 1-Ethyl-3-methylimidazolium methylphosphonate was used as the polar IL. As a model of partly depolymerised cellulose, a mixed sample composed of cellulose and cello-oligosaccharides (glucose, cellobiose, cellotetraose, and cellohexaose) was evaluated to test the resolution of the HPILC. In the resulting chromatograms, the corresponding peaks for each saccharide were found. Hydrolysed cellulose catalysed by a cellulase mixture in water was then prepared and dried. Then, this was dissolved in the polar IL to analyse its molecular weight distribution. The molecular weight distribution changed depending on the enzymatic reaction time. The peak for cellulose was found to decrease with the increase of the peak for cellobiose, and subsequently the peak for cellobiose decreased with the increase of that for glucose. In addition, cellulose oligomers except for cellobiose were scarcely observed, showing the catalytic feature of cellulase. Depolymerisation of cellulose in the polar IL was also carried out using ultrasonication. The peak for cellulose in the HPILC profiles shifted to a higher retention volume side and broadened with the sonication time, strongly suggesting random depolymerisation of cellulose. Thus, HPILC was confirmed to be effective for the dynamic analysis of cellulose depolymerisation. © The Royal Society of Chemistry 2013. Embargo Period 12 months Royal Society of Chemistry 2013-07-07 2017-10-03 eng journal article AM https://doi.org/10.24517/00009618 http://hdl.handle.net/2297/46349 https://kanazawa-u.repo.nii.ac.jp/records/9631 10.24517/00009618 10.1039/c3ay40182k 1759-9660 Analytical Methods 5 13 3172 3176 https://kanazawa-u.repo.nii.ac.jp/record/9631/files/TE-PR-KURODA-K-3172.pdf application/pdf 284.7 kB 2017-10-03