2026-03-11T13:39:30Z https://kanazawa-u.repo.nii.ac.jp/oai

oai:kanazawa-u.repo.nii.ac.jp:00029350 2024-06-25T06:32:46Z 1979:1980:1981

Static and dynamic hypergravity responses of osteoblasts and osteoclasts in medaka scales Yano, Sachiko 50443 Kitamura, Kei-ichiro 324 80283117 80283117 80283117 Satoh, Yusuke 50444 Nakano, Masaki 50445 Hattori, Atsuhiko 50446 Sekiguchi, Toshio 50447 Ikegame, Mika 50448 Nakashima, Hiroshi 50449 20020026 Omori, Katsunori 50450 Hayakawa, Kazuichi 35 40115267 40115267 40115267 Chiba, Atsuhiko 50451 Sasayama, Yuichi 15004 30018999 30018999 Ejiri, Sadakazu 50452 Mikuni-Takagaki, Yuko 50453 Mishima, Hiroyuki 50454 Funahashi, Hisayuki 50455 Sakamoto, Tatsuya 50456 Suzuki, Nobuo 26423 60242476 60242476 60242476 Fish scales are a form of calcified tissue similar to that found in human bone. In medaka scales, we detected both osteoblasts and osteoclasts and subsequently developed a new scale assay system. Using this system, we analyzed the osteoblastic and osteoclastic responses under 2-, 3-, and 4-gravity (G) loading by both centrifugation and vibration. After loading for 10 min, the scales from centrifugal and vibration loading were incubated for 6 and 24 hrs, respectively, after which the osteoblastic and osteoclastic activities were measured. Osteoblastic activity significantly increased under 2- to 4-G loading by both centrifugation and vibration. In contrast, we found that osteoclastic activity significantly decreased under 2- and 3-G loading in response to both centrifugation and vibration. Under 4-G loading, osteoclastic activity also decreased on centrifugation, but significantly increased under 4-G loading by vibration, concomitant with markedly increased osteoblastic activity. Expression of the receptor activator of the NF-αB ligand (RANKL), an activation factor of osteoclasts expressed in osteoblasts, increased significantly under 4-G loading by vibration but was unchanged by centrifugal loading. A protein sequence similar to osteoprotegerin (OPG), which is known as an osteoclastogenesis inhibitory factor, was found in medaka using our sequence analysis. The ratio of RANKL/OPG-like mRNAs in the vibration-loaded scales was significantly higher than that in the control scales, although there was no difference between centrifugal loaded scales and the control scales. Accordingly, medaka scales provide a useful model by which to analyze bone metabolism in response to physical strain. © 2013 Zoological Society of Japan. journal article Zoological Society of Japan = 日本動物学会 2013-03-01 VoR application/pdf Zoological Science 3 30 217 223 AA10545874 0289-0003 https://kanazawa-u.repo.nii.ac.jp/record/29350/files/NA-PR-YANO-SUZUMKI-217.pdf http://hdl.handle.net/2297/34731 https://kanazawa-u.repo.nii.ac.jp/records/29350 eng 10.2108/zsj.30.217 http://www.zoology.or.jp/ © 2013 Zoological Society of Japan