{"created":"2023-07-27T06:26:25.690472+00:00","id":10135,"links":{},"metadata":{"_buckets":{"deposit":"81a08215-6dc8-4744-a4a2-2b4f11343f11"},"_deposit":{"created_by":3,"id":"10135","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"10135"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00010135","sets":["934:935:937"]},"author_link":["15062","15064","15066","15065","15067","15068","15063"],"item_4_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2008-02-15","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"442","bibliographicPageStart":"433","bibliographicVolumeNumber":"314","bibliographic_titles":[{"bibliographic_title":"Developmental Biology"}]}]},"item_4_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The canonical Wnt pathway plays a central role in specifying vegetal cell fate in sea urchin embryos. SpKrl has been cloned as a direct target of nuclear β-catenin. Using Hemicentrotus pulcherrimus embryos, here we show that HpKrl controls the specification of secondary mesenchyme cells (SMCs) through both cell-autonomous and non-autonomous means. Like SpKrl, HpKrl was activated in both micromere and macromere progenies. To examine the functions of HpKrl in each blastomere, we constructed chimeric embryos composed of blastomeres from control and morpholino-mediated HpKrl-knockdown embryos and analyzed the phenotypes of the chimeras. Micromere-swapping experiments showed that HpKrl is not involved in micromere specification, while micromere-deprivation assays indicated that macromeres require HpKrl for cell-autonomous specification. Transplantation of normal micromeres into a micromere-less host with morpholino revealed that macromeres are able to receive at least some micromere signals regardless of HpKrl function. From these observations, we propose that two distinct pathways of endomesoderm formation exist in macromeres, a Krl-dependent pathway and a Krl-independent pathway. The Krl-independent pathway may correspond to the Delta/Notch signaling pathway via GataE and Gcm. We suggest that Krl may be a downstream component of nuclear β-catenin required by macromeres for formation of more vegetal tissues, not as a member of the Delta/Notch pathway, but as a parallel effector of the signaling (Krl-dependent pathway). © 2007 Elsevier Inc. All rights reserved.","subitem_description_type":"Abstract"}]},"item_4_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学大学院自然科学研究科遺伝情報学","subitem_description_type":"Other"}]},"item_4_publisher_17":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier"}]},"item_4_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.ydbio.2007.11.035","subitem_relation_type_select":"DOI"}}]},"item_4_source_id_11":{"attribute_name":"NCID","attribute_value_mlt":[{"subitem_source_identifier":"AA00627848","subitem_source_identifier_type":"NCID"}]},"item_4_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0012-1606","subitem_source_identifier_type":"ISSN"}]},"item_4_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Yamazaki, Atsuko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kawabata, Rika"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Shiomi, Kosuke"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tsuchimoto, Jun"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kiyomoto, Masato"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Amemiya, Shonan"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yamaguchi, Masaaki"}],"nameIdentifiers":[{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-10-03"}],"displaytype":"detail","filename":"SC-PR-YAMAGUCHI-M-433.pdf","filesize":[{"value":"850.0 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"SC-PR-YAMAGUCHI-M-433.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/10135/files/SC-PR-YAMAGUCHI-M-433.pdf"},"version_id":"c9c29db5-6d16-46fa-9df3-d3f0fe961325"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Krüppel-like is required for nonskeletogenic mesoderm specification in the sea urchin embryo","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Krüppel-like is required for nonskeletogenic mesoderm specification in the sea urchin embryo"}]},"item_type_id":"4","owner":"3","path":["937"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-10-03"},"publish_date":"2017-10-03","publish_status":"0","recid":"10135","relation_version_is_last":true,"title":["Krüppel-like is required for nonskeletogenic mesoderm specification in the sea urchin embryo"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-07-28T01:43:24.066469+00:00"}