{"created":"2023-07-27T06:26:26.544860+00:00","id":10156,"links":{},"metadata":{"_buckets":{"deposit":"00a3671f-10bd-4619-a903-707cf8316785"},"_deposit":{"created_by":3,"id":"10156","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"10156"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00010156","sets":["934:935:937"]},"author_link":["15111","15110","15055"],"item_4_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2002-01-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"132","bibliographicPageStart":"123","bibliographicVolumeNumber":"1597","bibliographic_titles":[{"bibliographic_title":"Biochimica et biophysica acta. Protein structure and molecular enzymology"}]}]},"item_4_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Ferredoxin–NAD(P)+ reductase [EC 1.18.1.3, 1.18.1.2] was isolated from the green sulfur bacterium Chlorobium tepidum and purified to homogeneity. The molecular mass of the subunit is 42 kDa, as deduced by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The molecular mass of the native enzyme is approximately 90 kDa, estimated by gel-permeation chromatography, and is thus a homodimer. The enzyme contains one FAD per subunit and has absorption maxima at about 272, 385, and 466 nm. In the presence of ferredoxin (Fd) and reaction center (RC) complex from C. tepidum, it efficiently catalyzes photoreduction of both NADP+ and NAD+. When concentrations of NADP+ exceeded 10 μM, NADP+ photoreduction rates decreased with increased concentration. The inhibition by high concentrations of substrate was not observed with NAD+. It also reduces 2,6-dichlorophenol-indophenol (DPIP) and molecular oxygen with either NADPH or NADH as efficient electron donors. It showed NADPH diaphorase activity about two times higher than NADH diaphorase activity in DPIP reduction assays at NAD(P)H concentrations less than 0.1 mM. At 0.5 mM NAD(P)H, the two activities were about the same, and at 1 mM, the former activity was slightly lower than the latter.","subitem_description_type":"Abstract"}]},"item_4_publisher_17":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier Biomedical Press"}]},"item_4_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1016/s0167-4838(02)00269-8","subitem_relation_type_select":"DOI"}}]},"item_4_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0167-4838","subitem_source_identifier_type":"ISSN"}]},"item_4_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"瀨尾, 悌介"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Seo, Daisuke"}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"Sakurai, Hidehiro"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-10-03"}],"displaytype":"detail","filename":"SC-PR-SEO-T-BBA 2002 .pdf","filesize":[{"value":"720.6 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"SC-PR-SEO-T-BBA 2002 .pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/10156/files/SC-PR-SEO-T-BBA 2002 .pdf"},"version_id":"713d5ea0-388e-450c-849a-504864855e1f"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Purification and characterization of ferredoxin–NAD(P)+ reductase from the green sulfur bacterium Chlorobium tepidum","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Purification and characterization of ferredoxin–NAD(P)+ reductase from the green sulfur bacterium Chlorobium tepidum"}]},"item_type_id":"4","owner":"3","path":["937"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-10-03"},"publish_date":"2017-10-03","publish_status":"0","recid":"10156","relation_version_is_last":true,"title":["Purification and characterization of ferredoxin–NAD(P)+ reductase from the green sulfur bacterium Chlorobium tepidum"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-07-27T11:31:57.699509+00:00"}