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Binary fission and encystation of Opalina sp. in axenic medium
http://hdl.handle.net/2297/9741
http://hdl.handle.net/2297/97418e4957ce-1073-4d1d-9b40-6de37bca9025
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
SC-PR-HIGASHI-H-381.pdf (327.6 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2017-10-03 | |||||
| タイトル | ||||||
| タイトル | Binary fission and encystation of Opalina sp. in axenic medium | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
Hanamura, Kentarou
× Hanamura, Kentarou× Higashi(Endoh), Hiroshi |
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| 提供者所属 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 金沢大学理工研究域自然システム学系 | |||||
| 書誌情報 |
Zoological Science 巻 18, 号 3, p. 381-387, 発行日 2001-01-01 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0289-0003 | |||||
| NCID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA10545874 | |||||
| DOI | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.2108/zsj.18.381 | |||||
| 出版者 | ||||||
| 出版者 | 日本動物学会 | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Opalina sp. were cultivated under an anaerobic condition in the complex medium containing DME/F-12 HAM mixture (Sigma) as a basic medium, and fetal bovine serum and autoclaved rumen fluid as additional components. In the medium, the opalinid cells reproduced by binary fission, and in the longest case they were maintained for more than one month, following successive transfers. When cells collected from the host in breeding season were cultured in the same medium, encystation occurred after rapid cell divisions. In this case, the population obtained from the frog had contained a sub-population of cells with small number of nuclei and small cell size. The sub-population might have been in the phase of palintomy that is a rapid sequence of binary fissions without cell growth and finally produces small cysts. Our successful maintenance of the opalinid cells can offer the first step for the establishment of the in-vitro culture method. | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
