@article{oai:kanazawa-u.repo.nii.ac.jp:00010330,
 author = {板垣, 英治 and Itagaki, Eiji and Matsushita, Hiroyuki and Hatta, Takashi},
 issue = {1},
 journal = {Journal of Biochemistry},
 month = {Jan},
 note = {3-Ketosteroid-Δ1-dehydrogenase from Nocardia corallina catalyzes transhydrogenation of 3-keto-4-ene-steroid to 3-keto-1,4-diene-steroid e.g., progesterone to 1,4-androstadiene-3,17-dione. The reaction proceeded linearly at first and then soon slowed down owing to equilibration. The turnover number of this reaction was of the same magnitude as that of the dehydrogenation of 3-keto-4-ene-steroid. The pH optimum was 8.4, which is lower than that of the dehydrogenase reaction. The enzyme has a wide specificity for hydrogen acceptor steroids. The K(m)' and K(max)' values for these steroids and the values of the corresponding 3-keto-4-ene-steroids were compared. Kinetic studies of the steroid transhydrogenase reaction demonstrated a typical ping-pong mechanism. The enzyme oxidized 1,2-tritiated progesterone and transferred the tritium atoms to the reaction product, 4-androstene-3,17-dione, and water. Transhydrogenation in D2O resulted in the incorporation of a deuterium atom into the C2-position of 4-androstene-3,17-dione. The results indicate that the enzyme catalyzes C1,C2-trans axial abstraction of hydrogen atoms from progesterone, transfer of the 1α-hydrogen to the C1-position of 1,4-androstadiene-3,17-dione and release of the 2β-hydrogen to water. Reaction schemes based on the experimental results are proposed. The enzyme also catalyzes the reduction of 3-keto-1,4-diene-steroids with reduced benzoyl viologen., 金沢大学自然科学研究科, 金沢大学理工研究域自然システム学系},
 pages = {122--127},
 title = {Steroid transhydrogenase activity of 3-ketosteroid-Δ1-dehydrogenase from Nocardia corallina},
 volume = {108},
 year = {1990}
}