{"created":"2023-07-27T06:26:34.003465+00:00","id":10335,"links":{},"metadata":{"_buckets":{"deposit":"6d984ba7-d176-4a8f-a67a-2cc4660b0a57"},"_deposit":{"created_by":3,"id":"10335","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"10335"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00010335","sets":["934:935:937"]},"author_link":["15557","15558","15560","15561","15559"],"item_4_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2001-12-24","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"7","bibliographicPageEnd":"1356","bibliographicPageStart":"1345","bibliographicVolumeNumber":"155","bibliographic_titles":[{"bibliographic_title":"Journal of Cell Biology"}]}]},"item_4_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Membrane-type 1 matrix metalloproteinase (MT1-MMP) is an integral membrane proteinase that degrades the pericellular extracellular matrix (ECM) and is expressed in many migratory cells, including invasive cancer cells. MT1-MMP has been shown to localize at the migration edge and to promote cell migration; however, it is not clear how the enzyme is regulated during the migration process. Here, we report that MT1-MMP is internalized from the surface and that this event depends on the sequence of its cytoplasmic tail. Di-leucine (Leu571–572 and Leu578–579) and tyrosine573 residues are important for the internalization, and the µ2 subunit of adaptor protein 2, a component of clathrin-coated pits for membrane protein internalization, was found to bind to the LLY573 sequence. MT1-MMP was internalized predominantly at the adherent edge and was found to colocalize with clathrin-coated vesicles. The mutations that disturb internalization caused accumulation of the enzyme at the adherent edge, though the net proteolytic activity was not affected much. Interestingly, whereas expression of MT1-MMP enhances cell migration and invasion, the internalization-defective mutants failed to promote either activity. These data indicate that dynamic turnover of MT1-MMP at the migration edge by internalization is important for proper enzyme function during cell migration and invasion.","subitem_description_type":"Abstract"}]},"item_4_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学自然科学研究科 理化学研究所・横浜研究所　免疫アレルギー科学総合研究センター(RCAI) 横浜市立大学大学院国際総合科学研究科生体超分子科学専攻　客員教授","subitem_description_type":"Other"}]},"item_4_publisher_17":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Rockefeller University Press"}]},"item_4_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1083/jcb.200108112","subitem_relation_type_select":"DOI"}}]},"item_4_source_id_11":{"attribute_name":"NCID","attribute_value_mlt":[{"subitem_source_identifier":"AA00694812","subitem_source_identifier_type":"NCID"}]},"item_4_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0021-9525","subitem_source_identifier_type":"ISSN"}]},"item_4_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Uekita, Takamasa"}],"nameIdentifiers":[{"nameIdentifier":"15557","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Itoh, Yoshifumi"}],"nameIdentifiers":[{"nameIdentifier":"15558","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yana, Ikuo"}],"nameIdentifiers":[{"nameIdentifier":"15559","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ohno, Hiroshi"}],"nameIdentifiers":[{"nameIdentifier":"15560","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"50233226","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=50233226"},{"nameIdentifier":"50233226","nameIdentifierScheme":"研究者番号","nameIdentifierURI":"https://nrid.nii.ac.jp/nrid/1000050233226"}]},{"creatorNames":[{"creatorName":"Seiki, Motoharu"}],"nameIdentifiers":[{"nameIdentifier":"15561","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"10154634","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=10154634"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-10-03"}],"displaytype":"detail","filename":"NS-PR-OHNO-M-1356.pdf","filesize":[{"value":"500.7 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"NS-PR-OHNO-M-1356.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/10335/files/NS-PR-OHNO-M-1356.pdf"},"version_id":"3f08d535-9f2a-4b3e-83f3-034afb34f056"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Cytoplasmic tail-dependent internalization of membrane-type 1 matrix metalloproteinase is important for its invasion-promoting activity","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Cytoplasmic tail-dependent internalization of membrane-type 1 matrix metalloproteinase is important for its invasion-promoting activity"}]},"item_type_id":"4","owner":"3","path":["937"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-10-03"},"publish_date":"2017-10-03","publish_status":"0","recid":"10335","relation_version_is_last":true,"title":["Cytoplasmic tail-dependent internalization of membrane-type 1 matrix metalloproteinase is important for its invasion-promoting activity"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2024-06-20T06:02:49.298717+00:00"}